Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins

Autor: Yu Murakami, Satoshi Ansai, Akari Yonemura, Masato Kinoshita
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Bio-Protocol, Vol 7, Iss 24 (2017)
Druh dokumentu: article
ISSN: 2331-8325
DOI: 10.21769/BioProtoc.2665
Popis: This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later than F1 generation.
Databáze: Directory of Open Access Journals