Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins
Autor: | Yu Murakami, Satoshi Ansai, Akari Yonemura, Masato Kinoshita |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: | |
Zdroj: | Bio-Protocol, Vol 7, Iss 24 (2017) |
Druh dokumentu: | article |
ISSN: | 2331-8325 |
DOI: | 10.21769/BioProtoc.2665 |
Popis: | This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later than F1 generation. |
Databáze: | Directory of Open Access Journals |
Externí odkaz: |
Pro tento záznam nejsou dostupné žádné jednotky.