Lactobacillus paracasei feeding improves immune control of influenza infection in mice
| Autor: | Belkacem, Nouria, Serafini, Nicolas, Wheeler, Richard, Derrien, Muriel, Boucinha, Lilia, Couesnon, Aurélie, Cerf-Bensussan, Nadine, Gomperts Boneca, Ivo, Di Santo, James, Taha, Muhamed-Kheir, Bourdet-Sicard, Raphaelle, Sun, Jie |
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| Přispěvatelé: | Infections Bactériennes Invasives, Institut Pasteur [Paris], Immunité Innée - Innate Immunity, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell Wall (BGPB), Danone Nutricia Research, BIOASTER Microbiology Technology Institute [Lyon], Laboratory of Intestinal Immunity (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence des Méningocoques et Haemophilus influenzae - National Reference Center Meningococci and Haemophilus influenzae (CNR), Danone Research, Groupe DANONE, The study was supported by the following grants: CI IMMUNOBIOTIC 1307014/00 IRT BAP301, Danone 30000221 as well as by the Institut Pasteur. In addition to funding, Danone Nutricia Research and Bioaster provided coordination and management. In addition, Danone Nutricia Research made available Lactobacilli strains and performed Microbiota analysis. The funders had no role in study design, decision to publish, or preparation of the manuscript. MD and RBS are employed by Danone Nutricia Research. Danone Nutricia Research provided support in the form of salaries for authors MD and RBS, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), TAHA, Muhamed-Kheir |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Bacterial Diseases
Viral Diseases Physiology Polymers MESH: Orthomyxoviridae Infections/immunology Colony Count Microbial lcsh:Medicine MESH: Orthomyxoviridae/immunology Pathology and Laboratory Medicine Mice [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Immune Physiology Medicine and Health Sciences MESH: Orthomyxoviridae Infections/prevention & control MESH: Animals MESH: Respiratory Tract Infections/prevention & control lcsh:Science Immune Response Respiratory Tract Infections Innate Immune System Immunity Cellular Mice Inbred BALB C food and beverages Animal Models Lacticaseibacillus paracasei Orthomyxoviridae Chemistry Infectious Diseases Experimental Organism Systems Macromolecules Physical Sciences [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Cytokines Female Research Article Infectious Disease Control Materials by Structure Immunology Materials Science MESH: Mice Inbred BALB C Mouse Models MESH: Probiotics/administration & dosage Research and Analysis Methods Microbiology Model Organisms Signs and Symptoms Orthomyxoviridae Infections Diagnostic Medicine Virology Prevotella Infection Animals MESH: Colony Count Microbial MESH: Mice MESH: Lactobacillus paracasei/physiology Inflammation Probiotics MESH: Respiratory Tract Infections/microbiology lcsh:R Biology and Life Sciences MESH: Respiratory Tract Infections/immunology Molecular Development Peptidoglycans Polymer Chemistry Influenza MESH: Orthomyxoviridae Infections/microbiology [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition Immune System lcsh:Q MESH: Immunity Cellular/immunology MESH: Female [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Viral Transmission and Infection Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2017, 12 (9), pp.e0184976. ⟨10.1371/journal.pone.0184976⟩ PLoS ONE, 2017, 12 (9), pp.e0184976. ⟨10.1371/journal.pone.0184976⟩ PLoS ONE, Vol 12, Iss 9, p e0184976 (2017) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0184976⟩ |
| Popis: | International audience; Respiratory tract infections such as flu cause severe morbidity and mortality and are among the leading causes of death in children and adults worldwide. Commensal microbiota is critical for orchestrating tissue homeostasis and immunity in the intestine. Probiotics represent an interesting source of immune modulators and several clinical studies have addressed the potential beneficial effects of probiotics against respiratory infections. Therefore, we have investigated the mechanisms of protection conferred by L. paracasei CNCM I-1518 strain in a mouse model of influenza infection. Notably, local myeloid cells accumulation is generated in the lungs after seven days feeding with L. paracasei prior to viral infection. L. paracasei-fed mice showed reduced susceptibility to the influenza infection, associated with less accumulation of inflammatory cells in the lungs, faster viral clearance and general health improvement. Interestingly, Allobaculum was significantly increased in L. paracasei-fed mice 7 days after influenza infection, even if the gut microbiota composition was not altered overall. L. paracasei-purified peptidoglycan partially recapitulated the protective phenotype observed with the entire bacteria. Collectively, our results demonstrate that oral consumption of L. paracasei CNCM I-1518 modulates lung immunity was associated with an improved control of influenza infection. These results further extend the beneficial role for certain lactobacilli to alleviate the burden of respiratory tract infections. |
| Databáze: | OpenAIRE |
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