Lactobacillus paracasei feeding improves immune control of influenza infection in mice

Autor: Belkacem, Nouria, Serafini, Nicolas, Wheeler, Richard, Derrien, Muriel, Boucinha, Lilia, Couesnon, Aurélie, Cerf-Bensussan, Nadine, Gomperts Boneca, Ivo, Di Santo, James, Taha, Muhamed-Kheir, Bourdet-Sicard, Raphaelle, Sun, Jie
Přispěvatelé: Infections Bactériennes Invasives, Institut Pasteur [Paris], Immunité Innée - Innate Immunity, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell Wall (BGPB), Danone Nutricia Research, BIOASTER Microbiology Technology Institute [Lyon], Laboratory of Intestinal Immunity (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence des Méningocoques et Haemophilus influenzae - National Reference Center Meningococci and Haemophilus influenzae (CNR), Danone Research, Groupe DANONE, The study was supported by the following grants: CI IMMUNOBIOTIC 1307014/00 IRT BAP301, Danone 30000221 as well as by the Institut Pasteur. In addition to funding, Danone Nutricia Research and Bioaster provided coordination and management. In addition, Danone Nutricia Research made available Lactobacilli strains and performed Microbiota analysis. The funders had no role in study design, decision to publish, or preparation of the manuscript. MD and RBS are employed by Danone Nutricia Research. Danone Nutricia Research provided support in the form of salaries for authors MD and RBS, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), TAHA, Muhamed-Kheir
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Bacterial Diseases
Viral Diseases
Physiology
Polymers
MESH: Orthomyxoviridae Infections/immunology
Colony Count
Microbial

lcsh:Medicine
MESH: Orthomyxoviridae/immunology
Pathology and Laboratory Medicine
Mice
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Immune Physiology
Medicine and Health Sciences
MESH: Orthomyxoviridae Infections/prevention & control
MESH: Animals
MESH: Respiratory Tract Infections/prevention & control
lcsh:Science
Immune Response
Respiratory Tract Infections
Innate Immune System
Immunity
Cellular

Mice
Inbred BALB C

food and beverages
Animal Models
Lacticaseibacillus paracasei
Orthomyxoviridae
Chemistry
Infectious Diseases
Experimental Organism Systems
Macromolecules
Physical Sciences
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Cytokines
Female
Research Article
Infectious Disease Control
Materials by Structure
Immunology
Materials Science
MESH: Mice
Inbred BALB C

Mouse Models
MESH: Probiotics/administration & dosage
Research and Analysis Methods
Microbiology
Model Organisms
Signs and Symptoms
Orthomyxoviridae Infections
Diagnostic Medicine
Virology
Prevotella Infection
Animals
MESH: Colony Count
Microbial

MESH: Mice
MESH: Lactobacillus paracasei/physiology
Inflammation
Probiotics
MESH: Respiratory Tract Infections/microbiology
lcsh:R
Biology and Life Sciences
MESH: Respiratory Tract Infections/immunology
Molecular Development
Peptidoglycans
Polymer Chemistry
Influenza
MESH: Orthomyxoviridae Infections/microbiology
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
Immune System
lcsh:Q
MESH: Immunity
Cellular/immunology

MESH: Female
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Viral Transmission and Infection
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2017, 12 (9), pp.e0184976. ⟨10.1371/journal.pone.0184976⟩
PLoS ONE, 2017, 12 (9), pp.e0184976. ⟨10.1371/journal.pone.0184976⟩
PLoS ONE, Vol 12, Iss 9, p e0184976 (2017)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0184976⟩
Popis: International audience; Respiratory tract infections such as flu cause severe morbidity and mortality and are among the leading causes of death in children and adults worldwide. Commensal microbiota is critical for orchestrating tissue homeostasis and immunity in the intestine. Probiotics represent an interesting source of immune modulators and several clinical studies have addressed the potential beneficial effects of probiotics against respiratory infections. Therefore, we have investigated the mechanisms of protection conferred by L. paracasei CNCM I-1518 strain in a mouse model of influenza infection. Notably, local myeloid cells accumulation is generated in the lungs after seven days feeding with L. paracasei prior to viral infection. L. paracasei-fed mice showed reduced susceptibility to the influenza infection, associated with less accumulation of inflammatory cells in the lungs, faster viral clearance and general health improvement. Interestingly, Allobaculum was significantly increased in L. paracasei-fed mice 7 days after influenza infection, even if the gut microbiota composition was not altered overall. L. paracasei-purified peptidoglycan partially recapitulated the protective phenotype observed with the entire bacteria. Collectively, our results demonstrate that oral consumption of L. paracasei CNCM I-1518 modulates lung immunity was associated with an improved control of influenza infection. These results further extend the beneficial role for certain lactobacilli to alleviate the burden of respiratory tract infections.
Databáze: OpenAIRE
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