| Autor: |
V, Quelch, K R, Reed, G W, Erdos, P L, Nicoletti, E M, Hoffmann |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
American journal of veterinary research. 45(7) |
| ISSN: |
0002-9645 |
| Popis: |
Phosphatidylcholine:cholesterol liposomes containing 4(5) carboxyfluorescein were modified by incorporation of Brucella abortus lipopolysaccharide. These vesicles were opsonized with bovine anti-B abortus or anti-B abortus plus complement. The fluorescent marker entrapped in antibody-opsonized liposomes was taken up by cultured bovine macrophages more effectively than was the same compound which had been loaded into nonopsonized liposomes. The addition of bovine complement to antibody-opsonized liposomes did not increase the extent of 4(5) carboxyfluorescein uptake over that caused by antibodies alone. The system, described in this report, could be used as a model for evaluation of the efficacy of selectively directing liposomes containing antibiotics to macrophages infected with intracellular B abortus, since macrophages possess Fc receptors capable of binding antibody-opsonized liposomes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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