In situ synthesis of peptide microarrays using ink-jet microdispensing

Autor:	Bogdan V, Antohe, Patrick W, Cooley
Rok vydání:	2007
Předmět:	Molecular Weight Mice Protein Array Analysis Animals Combinatorial Chemistry Techniques Colorimetry Enzyme-Linked Immunosorbent Assay Enkephalins Antibodies Chromatography High Pressure Liquid
Zdroj:	Methods in molecular biology (Clifton, N.J.). 381
ISSN:	1064-3745
Popis:	The study of protein-protein and protein-DNA interactions is critical to understand biological processes. This article presents the methodology to create peptide microarrays in situ for the high-throughput screening of complex biomolecules. The in situ ink-jet peptide synthesis results in a conservation of costly reagent and amino acids, whereas providing a means to produce denser peptide arrays. A smaller amount of test sample is required to observe interaction when using these high-density peptide arrays.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::dfcaf961b5a40b05058420efbbe7db40 https://pubmed.ncbi.nlm.nih.gov/17984526 Zobrazit plný text záznamu