[The staining of the central neurons in the pteropod mollusk Clione limacina with fluorescein-labelled alpha-bungarotoxin]

Autor:	L B, Popova, Iu V, Panchin
Rok vydání:	1998
Předmět:	Neurons Staining and Labeling Mollusca Animals Drug Interactions Fluorescein Receptors Cholinergic Bungarotoxins Coloring Agents Acetylcholine Ganglia Invertebrate
Zdroj:	Zhurnal vysshei nervnoi deiatelnosti imeni I P Pavlova. 48(2)
ISSN:	0044-4677
Popis:	FITC-labelled alpha-bungarotoxin (alpha BT[FITC]) was used to identify acetylcholine (ACh)-sensitive neurons in the central nervous system of pteropod mollusc Clione limacina. Two small symmetrical neurons were stained with alpha BT[FITC] on the dorsal surface of the cerebral ganglia. The staining was highly specific as it was completely blocked by preincubation in alpha-bungarotoxin [alpha BT] at very low concentration (10(-9) M). Nicotinic agonists and antagonists efficiently prevented alpha BT[FITC] staining while muscarinic agonists and antagonists had no effect. These findings confirm the suggestion that alpha BT[FITC] binds to nicotinic ACh receptors. The possible role is discussed of alpha BT[FITC]-stained cells in the complex of hunting behavior of Clione.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::844f18930b9faa57deea27602d60e385 https://pubmed.ncbi.nlm.nih.gov/9644817 Zobrazit plný text záznamu