| Autor: |
E S, Bochkareva, A, Horovitz, A S, Girshovich |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
The Journal of biological chemistry. 269(1) |
| ISSN: |
0021-9258 |
| Popis: |
The nonhydrolyzable ATP analogue ATP gamma S (adenosine 5'-3-O-(thio)triphosphate) is affinity cross-linked to GroEL by formation of a disulfide bridge in a peroxide-promoted reaction. By replacing with serine each of 3 cysteine residues in GroEL, it is shown that ATP gamma S specifically cross-links to Cys-137. It is thus demonstrated that the ATP bound to GroEL is in direct contact with Cys-137. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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