Direct demonstration that ATP is in contact with Cys-137 in chaperonin GroEL

Autor:	E S, Bochkareva, A, Horovitz, A S, Girshovich
Rok vydání:	1994
Předmět:	Adenosine Triphosphate Cross-Linking Reagents Bacterial Proteins Base Sequence Oligodeoxyribonucleotides Molecular Sequence Data Escherichia coli Chaperonin 60 Cysteine Heat-Shock Proteins
Zdroj:	The Journal of biological chemistry. 269(1)
ISSN:	0021-9258
Popis:	The nonhydrolyzable ATP analogue ATP gamma S (adenosine 5'-3-O-(thio)triphosphate) is affinity cross-linked to GroEL by formation of a disulfide bridge in a peroxide-promoted reaction. By replacing with serine each of 3 cysteine residues in GroEL, it is shown that ATP gamma S specifically cross-links to Cys-137. It is thus demonstrated that the ATP bound to GroEL is in direct contact with Cys-137.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::45f30fd5689a5a54487ef47af4977f52 https://pubmed.ncbi.nlm.nih.gov/7903969 Zobrazit plný text záznamu