Immunogenicity of Intensively Decellularized Equine Carotid Arteries Is Conferred by the Extracellular Matrix Protein Collagen Type VI
| Autor: | Heiko Meyer, Falk F. R. Buettner, Ulrike Boeer, Mathias Wilhelmi, Georgios C. Antonopoulos, Axel Haverich, Melanie Klingenberg |
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| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Pathology Cardiovascular Procedures immunogenicity Biochemistry Mice Tissue engineering Animal Cells Collagen VI lcsh:Science chemistry.chemical_classification epitope Tissue Scaffolds Stem Cells Immunogenicity horse tissue engineering Cytochemistry ddc:500 Cellular Structures and Organelles Cellular Types Collagen Type IV medicine.medical_specialty Immune Cells two dimensional gel electrophoresis Biomedical Engineering Bioengineering Surgical and Invasive Medical Procedures animal tissue Biomaterials biocompatibility liquid chromatography Horses cell protein xenograft mouse volumetry Blood Cells carotid artery lcsh:R Biology and Life Sciences Proteins Epithelial Cells DNA Equidae Biological Tissue chemistry smooth muscle actin Medical Devices and Equipment lcsh:Q Dewey Decimal Classification::600 | Technik::610 | Medizin Gesundheit transplantation lcsh:Medicine major histocompatibility antigen class 1 Matrix (biology) immunoprecipitation Epithelium immunology Extracellular matrix Medicine and Health Sciences deoxycholate sodium animal histocompatibility Dewey Decimal Classification::500 | Naturwissenschaften mass spectrometry antibody production Prosthetics Immune System Proteins Multidisciplinary Decellularization tissue scaffold scleroprotein article female Carotid Arteries Heterografts Anatomy Research Article Biotechnology extracellular matrix immunization Antibodies collagen type 4 Transplantation Immunology collagen type 6 parasitic diseases medicine Animals controlled study ddc:610 artery wall nonhuman Scleroprotein Cell Biology Molecular biology Transplantation DNA content protein analysis Clinical Immunology Pericytes clinical protocol |
| Zdroj: | PLoS ONE, Vol 9, Iss 8, p e105964 (2014) PLoS ONE PLoS ONE 9 (2014), Nr. 8 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0105964 |
| Popis: | The limited biocompatibility of decellularized scaffolds is an ongoing challenge in tissue engineering. Here, we demonstrate the residual immunogenicity of an extensively decellularized equine carotid artery (dEAC intens) and identify the involved immunogenic components. EAC were submitted to an elaborated intensified decellularization protocol with SDS/sodium desoxycholate for 72 h using increased processing volumes (dEAC intens), and compared to dEACord prepared by an ordinary protocol (40 h, normal volumes). Matrix integrity was checked via correlative volumetric visualization which revealed only minor structural changes in the arterial wall. In dEACintens, a substantial depletion of cellular components was obvious for smooth muscle actin (100%), MHC I complexes (97.8%), alphaGal epitops (98.4% and 91.3%) and for DNA (final concentration of 0.34±0.16 ng/mg tissue). However, dEACintens still evoked antibody formation in mice after immunization with dEACintens extracts, although to a lower extent than dEACord. Mouse plasma antibodies recognized a 140 kDa band which was revealed to contain collagen VI alpha1 and alpha2 chains via mass spectrometry of both 2D electrophoretically separated and immunoprecipitated proteins. Thus, even the complete removal of cellular proteins did not yield non-immunogenic dEAC as the extracellular matrix still conferred immunogenicity by collagen VI. However, as lower antibody levels were achieved by the intensified decellularization protocol, this seems to be a promising basis for further development. |
| Databáze: | OpenAIRE |
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