Immunogenicity of Intensively Decellularized Equine Carotid Arteries Is Conferred by the Extracellular Matrix Protein Collagen Type VI

Autor: Heiko Meyer, Falk F. R. Buettner, Ulrike Boeer, Mathias Wilhelmi, Georgios C. Antonopoulos, Axel Haverich, Melanie Klingenberg
Rok vydání: 2014
Předmět:
Proteomics
Pathology
Cardiovascular Procedures
immunogenicity
Biochemistry
Mice
Tissue engineering
Animal Cells
Collagen VI
lcsh:Science
chemistry.chemical_classification
epitope
Tissue Scaffolds
Stem Cells
Immunogenicity
horse
tissue engineering
Cytochemistry
ddc:500
Cellular Structures and Organelles
Cellular Types
Collagen Type IV
medicine.medical_specialty
Immune Cells
two dimensional gel electrophoresis
Biomedical Engineering
Bioengineering
Surgical and Invasive Medical Procedures
animal tissue
Biomaterials
biocompatibility
liquid chromatography
Horses
cell protein
xenograft
mouse
volumetry
Blood Cells
carotid artery
lcsh:R
Biology and Life Sciences
Proteins
Epithelial Cells
DNA
Equidae
Biological Tissue
chemistry
smooth muscle actin
Medical Devices and Equipment
lcsh:Q
Dewey Decimal Classification::600 | Technik::610 | Medizin
Gesundheit

transplantation
lcsh:Medicine
major histocompatibility antigen class 1
Matrix (biology)
immunoprecipitation
Epithelium
immunology
Extracellular matrix
Medicine and Health Sciences
deoxycholate sodium
animal
histocompatibility
Dewey Decimal Classification::500 | Naturwissenschaften
mass spectrometry
antibody production
Prosthetics
Immune System Proteins
Multidisciplinary
Decellularization
tissue scaffold
scleroprotein
article
female
Carotid Arteries
Heterografts
Anatomy
Research Article
Biotechnology
extracellular matrix
immunization
Antibodies
collagen type 4
Transplantation Immunology
collagen type 6
parasitic diseases
medicine
Animals
controlled study
ddc:610
artery wall
nonhuman
Scleroprotein
Cell Biology
Molecular biology
Transplantation
DNA content
protein analysis
Clinical Immunology
Pericytes
clinical protocol
Zdroj: PLoS ONE, Vol 9, Iss 8, p e105964 (2014)
PLoS ONE
PLoS ONE 9 (2014), Nr. 8
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0105964
Popis: The limited biocompatibility of decellularized scaffolds is an ongoing challenge in tissue engineering. Here, we demonstrate the residual immunogenicity of an extensively decellularized equine carotid artery (dEAC intens) and identify the involved immunogenic components. EAC were submitted to an elaborated intensified decellularization protocol with SDS/sodium desoxycholate for 72 h using increased processing volumes (dEAC intens), and compared to dEACord prepared by an ordinary protocol (40 h, normal volumes). Matrix integrity was checked via correlative volumetric visualization which revealed only minor structural changes in the arterial wall. In dEACintens, a substantial depletion of cellular components was obvious for smooth muscle actin (100%), MHC I complexes (97.8%), alphaGal epitops (98.4% and 91.3%) and for DNA (final concentration of 0.34±0.16 ng/mg tissue). However, dEACintens still evoked antibody formation in mice after immunization with dEACintens extracts, although to a lower extent than dEACord. Mouse plasma antibodies recognized a 140 kDa band which was revealed to contain collagen VI alpha1 and alpha2 chains via mass spectrometry of both 2D electrophoretically separated and immunoprecipitated proteins. Thus, even the complete removal of cellular proteins did not yield non-immunogenic dEAC as the extracellular matrix still conferred immunogenicity by collagen VI. However, as lower antibody levels were achieved by the intensified decellularization protocol, this seems to be a promising basis for further development.
Databáze: OpenAIRE