A reverse transcription-PCR assay to detect viable Mycoplasma synoviae in poultry environmental samples

Autor: Marylène Kobisch, C Savoye, Corinne Marois, Isabelle Kempf
Přispěvatelé: Kempf, Isabelle, Laboratoire d'études et de recherches avicoles, porcines et piscicoles, Agence Française de Sécurité Sanitaire des Aliments (AFSSA)
Rok vydání: 2002
Předmět:
Turkeys
Air Microbiology
Mycoplasmataceae
Mycoplasma synoviae
Biology
MESH: Disease Transmission
Infectious
MESH: Poultry Diseases
medicine.disease_cause
Microbiology
law.invention
MESH: Mycoplasma
Mycoplasma
law
MESH: Air Microbiology
MESH: Reverse Transcriptase Polymerase Chain Reaction
RNA
Ribosomal
16S
medicine
Disease Transmission
Infectious
Animals
MESH: Animals
Mycoplasma Infections
Pathogen
Polymerase chain reaction
Poultry Diseases
Soil Microbiology
MESH: Turkeys
General Veterinary
Reverse Transcriptase Polymerase Chain Reaction
MESH: Chickens
General Medicine
Ribosomal RNA
MESH: Mycoplasma Infections
16S ribosomal RNA
biology.organism_classification
Virology
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Water Microbiology
MESH: RNA
Ribosomal
16S
RNA
Bacterial
MESH: Soil Microbiology
Mollicutes
Female
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: RNA
Bacterial
Water Microbiology
MESH: Female
Chickens
Zdroj: Veterinary Microbiology
Veterinary Microbiology, Elsevier, 2002, 89 (1), pp.17-28
ISSN: 0378-1135
Popis: In order to study horizontal transmission of Mycoplasma synoviae an avian pathogen, a reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to detect viable Mycoplasma in environment. The test was based on the RT-PCR of the 16S ribosomal RNA (rRNA) of Mycoplasma genus. Results showed that Mycoplasma 16S rRNA was stable up to 23 h after cell death. Therefore, the test allowed detection of viable or very recently (less than 23 h) dead mycoplasmas. M. synoviae survival in artificially contaminated water, food and soil and in the environment of M. synoviae experimentally infected turkeys was estimated by culture and RT-PCR. The RT-PCR method was then applied in a naturally infected laying hen farm showing problems of recurrent mycoplasmosis in the hens. Results confirmed the usefulness of RT-PCR in checking the efficiency of biosecurity measures and in improving cleaning and disinfection protocols.
Databáze: OpenAIRE
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