A reverse transcription-PCR assay to detect viable Mycoplasma synoviae in poultry environmental samples
| Autor: | Marylène Kobisch, C Savoye, Corinne Marois, Isabelle Kempf |
|---|---|
| Přispěvatelé: | Kempf, Isabelle, Laboratoire d'études et de recherches avicoles, porcines et piscicoles, Agence Française de Sécurité Sanitaire des Aliments (AFSSA) |
| Rok vydání: | 2002 |
| Předmět: |
Turkeys
Air Microbiology Mycoplasmataceae Mycoplasma synoviae Biology MESH: Disease Transmission Infectious MESH: Poultry Diseases medicine.disease_cause Microbiology law.invention MESH: Mycoplasma Mycoplasma law MESH: Air Microbiology MESH: Reverse Transcriptase Polymerase Chain Reaction RNA Ribosomal 16S medicine Disease Transmission Infectious Animals MESH: Animals Mycoplasma Infections Pathogen Polymerase chain reaction Poultry Diseases Soil Microbiology MESH: Turkeys General Veterinary Reverse Transcriptase Polymerase Chain Reaction MESH: Chickens General Medicine Ribosomal RNA MESH: Mycoplasma Infections 16S ribosomal RNA biology.organism_classification Virology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Water Microbiology MESH: RNA Ribosomal 16S RNA Bacterial MESH: Soil Microbiology Mollicutes Female [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: RNA Bacterial Water Microbiology MESH: Female Chickens |
| Zdroj: | Veterinary Microbiology Veterinary Microbiology, Elsevier, 2002, 89 (1), pp.17-28 |
| ISSN: | 0378-1135 |
| Popis: | In order to study horizontal transmission of Mycoplasma synoviae an avian pathogen, a reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to detect viable Mycoplasma in environment. The test was based on the RT-PCR of the 16S ribosomal RNA (rRNA) of Mycoplasma genus. Results showed that Mycoplasma 16S rRNA was stable up to 23 h after cell death. Therefore, the test allowed detection of viable or very recently (less than 23 h) dead mycoplasmas. M. synoviae survival in artificially contaminated water, food and soil and in the environment of M. synoviae experimentally infected turkeys was estimated by culture and RT-PCR. The RT-PCR method was then applied in a naturally infected laying hen farm showing problems of recurrent mycoplasmosis in the hens. Results confirmed the usefulness of RT-PCR in checking the efficiency of biosecurity measures and in improving cleaning and disinfection protocols. |
| Databáze: | OpenAIRE |
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