Correlation between the pronucleus size and the potential for human single pronucleus zygotes to develop into blastocysts

Autor: Yukiko Asano, Kazuyo Nishikawa, Fumiaki Itoi, Hiroyuki Honnma, Eri Araki, Hisanori Oguri
Rok vydání: 2018
Předmět:
Zdroj: Journal of Assisted Reproduction and Genetics. 35:817-823
ISSN: 1573-7330
1058-0468
DOI: 10.1007/s10815-018-1137-1
Popis: PURPOSE: In this study, we examined the correlation between pronucleus size and the potential for human single pronucleus (1PN) zygotes to develop into blastocysts after IVF and ICSI. METHODS: This study included 112 patients who underwent a total of 112 cycles of IVF/ICSI. To evaluate embryo development, 1PN zygotes were compared with 2PN zygotes in the same IVF/ICSI cycle (control cycles) using time-lapse live embryo imaging. To assess the potential for blastocyst formation, cutoff values for pronuclear area and diameter were established through receiver operating characteristic curve analysis, after which 1PN zygotes were classified based on those cutoff values. RESULTS: Among 1PN zygotes cultured to day 5/6, the rate of embryo development was significantly lower than from 2PN zygotes. However, the rates of blastocyst formation and good quality blastocysts from 1PN zygotes with large pronuclear areas (≥ 710 μm(2)) or diameters (≥ 31 μm) were significantly higher than from 1PN zygotes with smaller pronuclear areas (≤ 509, 510–609, and 610–709 μm(2)) or diameters (≤ 24, 25–27,and 28–30 μm) (P
Databáze: OpenAIRE
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