Correlation between the pronucleus size and the potential for human single pronucleus zygotes to develop into blastocysts
| Autor: | Yukiko Asano, Kazuyo Nishikawa, Fumiaki Itoi, Hiroyuki Honnma, Eri Araki, Hisanori Oguri |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine animal structures Zygote Fertilization in Vitro Biology Time-Lapse Imaging Andrology 03 medical and health sciences 0302 clinical medicine Genetics medicine Humans Blastocyst reproductive and urinary physiology Genetics (clinical) Retrospective Studies Cell Nucleus 030219 obstetrics & reproductive medicine Pronucleus urogenital system Embryogenesis Obstetrics and Gynecology General Medicine Embryo Biology 030104 developmental biology medicine.anatomical_structure ROC Curve Reproductive Medicine embryonic structures Female therapeutics Developmental Biology |
| Zdroj: | Journal of Assisted Reproduction and Genetics. 35:817-823 |
| ISSN: | 1573-7330 1058-0468 |
| Popis: | PURPOSE: In this study, we examined the correlation between pronucleus size and the potential for human single pronucleus (1PN) zygotes to develop into blastocysts after IVF and ICSI. METHODS: This study included 112 patients who underwent a total of 112 cycles of IVF/ICSI. To evaluate embryo development, 1PN zygotes were compared with 2PN zygotes in the same IVF/ICSI cycle (control cycles) using time-lapse live embryo imaging. To assess the potential for blastocyst formation, cutoff values for pronuclear area and diameter were established through receiver operating characteristic curve analysis, after which 1PN zygotes were classified based on those cutoff values. RESULTS: Among 1PN zygotes cultured to day 5/6, the rate of embryo development was significantly lower than from 2PN zygotes. However, the rates of blastocyst formation and good quality blastocysts from 1PN zygotes with large pronuclear areas (≥ 710 μm(2)) or diameters (≥ 31 μm) were significantly higher than from 1PN zygotes with smaller pronuclear areas (≤ 509, 510–609, and 610–709 μm(2)) or diameters (≤ 24, 25–27,and 28–30 μm) (P |
| Databáze: | OpenAIRE |
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