Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm

Autor: Anne Beauvais, Jacques Guillot, Benoit Briard, Eric Dannaoui, Leslie Anaïs, Jean-Winoc Decousser, Elise Melloul, Jean-Marc Costa, Pascal Arné, Vincent Fihman, Stéphanie Luiggi, Françoise Botterel
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Hyphal growth
Microscopy
Electron
Scanning Transmission

Fungal Structure
Stenotrophomonas maltophilia
lcsh:Medicine
Pathology and Laboratory Medicine
Aspergillus fumigatus
Medicine and Health Sciences
lcsh:Science
DNA
Fungal

Fungal Pathogens
Multidisciplinary
biology
Chemistry
Fungal genetics
Bacterial Pathogens
Aspergillus
Medical Microbiology
Biological Cultures
Pathogens
Cellular Structures and Organelles
Research Article
Cell Culturing Techniques
DNA
Bacterial

Biofilm Culture
Hypha
030106 microbiology
Mycology
In Vitro Techniques
Research and Analysis Methods
Real-Time Polymerase Chain Reaction
Microbiology
Models
Biological

03 medical and health sciences
Cell Walls
Antibiosis
Microbial Pathogens
Microbial Viability
fungi
lcsh:R
Biofilm
Organisms
Fungi
Biology and Life Sciences
Bacteriology
Cell Biology
biochemical phenomena
metabolism
and nutrition

biology.organism_classification
Molds (Fungi)
030104 developmental biology
Biofilms
Microscopy
Electron
Scanning

lcsh:Q
Bacterial Biofilms
Bacteria
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 11, p e0166325 (2016)
ISSN: 1932-6203
Popis: Background Biofilms are communal structures of microorganisms that have long been associated with a variety of persistent infections poorly responding to conventional antibiotic or antifungal therapy. Aspergillus fumigatus fungus and Stenotrophomonas maltophilia bacteria are examples of the microorganisms that can coexist to form a biofilm especially in the respiratory tract of immunocompromised patients or cystic fibrosis patients. The aim of the present study was to develop and assess an in vitro model of a mixed biofilm associating S. maltophilia and A. fumigatus by using analytical and quantitative approaches. Materials and Methods An A. fumigatus strain (ATCC 13073) expressing a Green Fluorescent Protein (GFP) and an S. maltophilia strain (ATCC 13637) were used. Fungal and bacterial inocula (105 conidia/mL and 106 cells/mL, respectively) were simultaneously deposited to initiate the development of an in vitro mixed biofilm on polystyrene supports at 37°C for 24 h. The structure of the biofilm was analysed via qualitative microscopic techniques like scanning electron and transmission electron microscopy, and fluorescence microscopy, and by quantitative techniques including qPCR and crystal violet staining. Results Analytic methods revealed typical structures of biofilm with production of an extracellular matrix (ECM) enclosing fungal hyphae and bacteria. Quantitative methods showed a decrease of A. fumigatus growth and ECM production in the mixed biofilm with antibiosis effect of the bacteria on the fungi seen as abortive hyphae, limited hyphal growth, fewer conidia, and thicker fungal cell walls. Conclusion For the first time, a mixed A. fumigatus—S. maltophilia biofilm was validated by various analytical and quantitative approaches and the bacterial antibiosis effect on the fungus was demonstrated. The mixed biofilm model is an interesting experimentation field to evaluate efficiency of antimicrobial agents and to analyse the interactions between the biofilm and the airways epithelium.
Databáze: OpenAIRE
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