Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm
| Autor: | Anne Beauvais, Jacques Guillot, Benoit Briard, Eric Dannaoui, Leslie Anaïs, Jean-Winoc Decousser, Elise Melloul, Jean-Marc Costa, Pascal Arné, Vincent Fihman, Stéphanie Luiggi, Françoise Botterel |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hyphal growth Microscopy Electron Scanning Transmission Fungal Structure Stenotrophomonas maltophilia lcsh:Medicine Pathology and Laboratory Medicine Aspergillus fumigatus Medicine and Health Sciences lcsh:Science DNA Fungal Fungal Pathogens Multidisciplinary biology Chemistry Fungal genetics Bacterial Pathogens Aspergillus Medical Microbiology Biological Cultures Pathogens Cellular Structures and Organelles Research Article Cell Culturing Techniques DNA Bacterial Biofilm Culture Hypha 030106 microbiology Mycology In Vitro Techniques Research and Analysis Methods Real-Time Polymerase Chain Reaction Microbiology Models Biological 03 medical and health sciences Cell Walls Antibiosis Microbial Pathogens Microbial Viability fungi lcsh:R Biofilm Organisms Fungi Biology and Life Sciences Bacteriology Cell Biology biochemical phenomena metabolism and nutrition biology.organism_classification Molds (Fungi) 030104 developmental biology Biofilms Microscopy Electron Scanning lcsh:Q Bacterial Biofilms Bacteria |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 11, p e0166325 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Background Biofilms are communal structures of microorganisms that have long been associated with a variety of persistent infections poorly responding to conventional antibiotic or antifungal therapy. Aspergillus fumigatus fungus and Stenotrophomonas maltophilia bacteria are examples of the microorganisms that can coexist to form a biofilm especially in the respiratory tract of immunocompromised patients or cystic fibrosis patients. The aim of the present study was to develop and assess an in vitro model of a mixed biofilm associating S. maltophilia and A. fumigatus by using analytical and quantitative approaches. Materials and Methods An A. fumigatus strain (ATCC 13073) expressing a Green Fluorescent Protein (GFP) and an S. maltophilia strain (ATCC 13637) were used. Fungal and bacterial inocula (105 conidia/mL and 106 cells/mL, respectively) were simultaneously deposited to initiate the development of an in vitro mixed biofilm on polystyrene supports at 37°C for 24 h. The structure of the biofilm was analysed via qualitative microscopic techniques like scanning electron and transmission electron microscopy, and fluorescence microscopy, and by quantitative techniques including qPCR and crystal violet staining. Results Analytic methods revealed typical structures of biofilm with production of an extracellular matrix (ECM) enclosing fungal hyphae and bacteria. Quantitative methods showed a decrease of A. fumigatus growth and ECM production in the mixed biofilm with antibiosis effect of the bacteria on the fungi seen as abortive hyphae, limited hyphal growth, fewer conidia, and thicker fungal cell walls. Conclusion For the first time, a mixed A. fumigatus—S. maltophilia biofilm was validated by various analytical and quantitative approaches and the bacterial antibiosis effect on the fungus was demonstrated. The mixed biofilm model is an interesting experimentation field to evaluate efficiency of antimicrobial agents and to analyse the interactions between the biofilm and the airways epithelium. |
| Databáze: | OpenAIRE |
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