Entrapping Ribosomes for Viral Translation

Autor: Hugo H. J. Bink, Cornelis W. A. Pleij, Barend Kraal, Sharief Barends, Sjoerd H. E. van den Worm
Rok vydání: 2003
Předmět:
Zdroj: Cell. 112:123-129
ISSN: 0092-8674
DOI: 10.1016/s0092-8674(02)01256-4
Popis: Turnip yellow mosaic virus (TYMV) has a genomic plus-strand RNA with a 5' cap followed by overlapping and different reading frames for the movement protein and polyprotein, while the distal coat protein cistron is translated from a subgenomic RNA. The 3'-untranslated region harbors a tRNA-like structure (TLS) to which a valine moiety can be added and it is indispensable for virus viability. Here, we report about a surprising interaction between TYMV-RNA-programmed ribosomes and 3'-valylated TLS that yields polyprotein with the valine N terminally incorporated by a translation mechanism resistant to regular initiation inhibitors. Disruption of the TLS exclusively abolishes polyprotein synthesis, which can be restored by adding excess TLS in trans. Our observations imply a novel eukaryotic mechanism for internal initiation of mRNA translation.
Databáze: OpenAIRE
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