| Autor: |
Hugo H. J. Bink, Cornelis W. A. Pleij, Barend Kraal, Sharief Barends, Sjoerd H. E. van den Worm |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Cell. 112:123-129 |
| ISSN: |
0092-8674 |
| DOI: |
10.1016/s0092-8674(02)01256-4 |
| Popis: |
Turnip yellow mosaic virus (TYMV) has a genomic plus-strand RNA with a 5' cap followed by overlapping and different reading frames for the movement protein and polyprotein, while the distal coat protein cistron is translated from a subgenomic RNA. The 3'-untranslated region harbors a tRNA-like structure (TLS) to which a valine moiety can be added and it is indispensable for virus viability. Here, we report about a surprising interaction between TYMV-RNA-programmed ribosomes and 3'-valylated TLS that yields polyprotein with the valine N terminally incorporated by a translation mechanism resistant to regular initiation inhibitors. Disruption of the TLS exclusively abolishes polyprotein synthesis, which can be restored by adding excess TLS in trans. Our observations imply a novel eukaryotic mechanism for internal initiation of mRNA translation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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