A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus
| Autor: | Chenghui Huang, Susan Yung, Gongping Liang, Qianjin Lu, Rong Xiao, Tak Mao Chan, Ming Zhao, Yaping Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male lcsh:Immunologic diseases. Allergy CD4-Positive T-Lymphocytes - immunology - metabolism Adolescent Article Subject Immunology Cell Cycle Proteins chemical and pharmacologic phenomena HMGB1 Young Adult immune system diseases medicine Humans Lupus Erythematosus Systemic Immunology and Allergy RNA Messenger HMGB1 Protein skin and connective tissue diseases Gene Regulation of gene expression Lupus erythematosus biology Nuclear Proteins General Medicine Middle Aged DNA Methylation Lupus Erythematosus Systemic - genetics - immunology - metabolism medicine.disease Molecular biology DNA demethylation Gene Expression Regulation Case-Control Studies DNA methylation biology.protein Female HMGB1 Protein - genetics - metabolism lcsh:RC581-607 GADD45A Research Article DNA hypomethylation |
| Zdroj: | Clinical and Developmental Immunology, Vol 2013 (2013) Clinical and Developmental Immunology |
| ISSN: | 1740-2530 1740-2522 |
| Popis: | The aberrant activity of CD4(+) T cells in patients with systemic lupus erythematosus (SLE) is associated with DNA hypomethylation of the regulatory regions in CD11a and CD70 genes. Our previous studies demonstrated that Gadd45a contributes to the development of SLE by promoting DNA demethylation in CD4(+) T cells. In this study, we identified proteins that bind to Gadd45a in CD4(+) T cells during SLE flare by using the method of co-immunoprecipitation and mass spectrometry, High mobility group box protein 1 (HMGB1) is one of identified proteins. Furthermore, gene and protein expression of HMGB1 was significantly increased in SLE CD4(+) T cells compared to controls, and HMGB1 mRNA was correlated with CD11a and CD70 mRNA. A significant, positive correlation was found between HMGB1 mRNA and SLEDAI for SLE patients. Our data demonstrate that HMGB1 binds to Gadd45a and may be involved in DNA demethylation in CD4(+) T cells during lupus flare. published_or_final_version |
| Databáze: | OpenAIRE |
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