Evidence for detrimental cross interactions between reactive oxygen and nitrogen species in Leber's hereditary optic neuropathy cells
| Autor: | Elena Forte, Maria Chiara Buscarinu, Kristina Radić, Giulia Coarelli, Paolo Sarti, Luciana Chessa, Micol Falabella, Marzia Arese, Alessandro Giuffrè, Maria Chiara Magnifico, Paolo Maria Santini, Rosella Mechelli, Marco Salvetti |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aging Pathology genetic structures medicine.disease_cause bioenergetics Biochemistry Optic neuropathy chemistry.chemical_compound Adenosine Triphosphate 0302 clinical medicine Fluorometry Lymphocytes chemistry.chemical_classification Leber's Hereditary Optic Neuropathy Reactive Oxygen and Nitrogen Species lcsh:Cytology Leber's hereditary optic neuropathy General Medicine Flow Cytometry Reactive Nitrogen Species nitrosative stress Female Research Article Adult Programmed cell death medicine.medical_specialty Article Subject Cell Survival Nitrogen Mitochondrial diseases Optic Atrophy Hereditary Leber Oxidative phosphorylation Biology Peripheral blood mononuclear cell pathogenic mechanisms 03 medical and health sciences Oxygen Consumption medicine Humans cell biology aging biochemistry lcsh:QH573-671 Nitrites Reactive nitrogen species Reactive oxygen species Cell Biology medicine.disease Molecular biology eye diseases Oxygen Oxidative Stress 030104 developmental biology chemistry Mutation Leukocytes Mononuclear Tyrosine Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Oxidative medicine and cellular longevity (Online) 2016 (2016): Article ID 3187560. doi:10.1155/2016/3187560 info:cnr-pdr/source/autori:Falabella, Micol; Forte, Elena; Magnifico, Maria Chiara; Santini, Paolo; Arese, Marzia; Giuffrè, Alessandro; Radic, Kristina; Chessa, Luciana; Coarelli, Giulia; Buscarinu, Maria Chiara; Mechelli, Rosella; Salvetti, Marco; Sarti, Paolo/titolo:Evidence for detrimental cross interactions between reactive oxygen and nitrogen species in Leber's hereditary optic neuropathy cells/doi:10.1155%2F2016%2F3187560/rivista:Oxidative medicine and cellular longevity (Online)/anno:2016/pagina_da:Article ID 3187560/pagina_a:/intervallo_pagine:Article ID 3187560/volume:2016 Oxidative Medicine and Cellular Longevity, Vol 2016 (2016) Oxidative Medicine and Cellular Longevity |
| DOI: | 10.1155/2016/3187560 |
| Popis: | Here we have collected evidence suggesting that chronic changes in the NO homeostasis and the rise of reactive oxygen species bioavailability can contribute to cell dysfunction in Leber’s hereditary optic neuropathy (LHON) patients. We report that peripheral blood mononuclear cells (PBMCs), derived from a female LHON patient with bilateral reduced vision and carrying the pathogenic mutation 11778/ND4, display increased levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS), as revealed by flow cytometry, fluorometric measurements of nitrite/nitrate, and 3-nitrotyrosine immunodetection. Moreover, viability assays with the tetrazolium dye MTT showed that lymphoblasts from the same patient are more sensitive to prolonged NO exposure, leading to cell death. Taken together these findings suggest that oxidative and nitrosative stress cooperatively play an important role in driving LHON pathology when excess NO remains available over time in the cell environment. |
| Databáze: | OpenAIRE |
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