Evidence for detrimental cross interactions between reactive oxygen and nitrogen species in Leber's hereditary optic neuropathy cells

Autor: Elena Forte, Maria Chiara Buscarinu, Kristina Radić, Giulia Coarelli, Paolo Sarti, Luciana Chessa, Micol Falabella, Marzia Arese, Alessandro Giuffrè, Maria Chiara Magnifico, Paolo Maria Santini, Rosella Mechelli, Marco Salvetti
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Aging
Pathology
genetic structures
medicine.disease_cause
bioenergetics
Biochemistry
Optic neuropathy
chemistry.chemical_compound
Adenosine Triphosphate
0302 clinical medicine
Fluorometry
Lymphocytes
chemistry.chemical_classification
Leber's Hereditary Optic Neuropathy
Reactive Oxygen and Nitrogen Species
lcsh:Cytology
Leber's hereditary optic neuropathy
General Medicine
Flow Cytometry
Reactive Nitrogen Species
nitrosative stress
Female
Research Article
Adult
Programmed cell death
medicine.medical_specialty
Article Subject
Cell Survival
Nitrogen
Mitochondrial diseases
Optic Atrophy
Hereditary
Leber
Oxidative phosphorylation
Biology
Peripheral blood mononuclear cell
pathogenic mechanisms
03 medical and health sciences
Oxygen Consumption
medicine
Humans
cell biology
aging
biochemistry
lcsh:QH573-671
Nitrites
Reactive nitrogen species
Reactive oxygen species
Cell Biology
medicine.disease
Molecular biology
eye diseases
Oxygen
Oxidative Stress
030104 developmental biology
chemistry
Mutation
Leukocytes
Mononuclear
Tyrosine
Reactive Oxygen Species
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Oxidative medicine and cellular longevity (Online) 2016 (2016): Article ID 3187560. doi:10.1155/2016/3187560
info:cnr-pdr/source/autori:Falabella, Micol; Forte, Elena; Magnifico, Maria Chiara; Santini, Paolo; Arese, Marzia; Giuffrè, Alessandro; Radic, Kristina; Chessa, Luciana; Coarelli, Giulia; Buscarinu, Maria Chiara; Mechelli, Rosella; Salvetti, Marco; Sarti, Paolo/titolo:Evidence for detrimental cross interactions between reactive oxygen and nitrogen species in Leber's hereditary optic neuropathy cells/doi:10.1155%2F2016%2F3187560/rivista:Oxidative medicine and cellular longevity (Online)/anno:2016/pagina_da:Article ID 3187560/pagina_a:/intervallo_pagine:Article ID 3187560/volume:2016
Oxidative Medicine and Cellular Longevity, Vol 2016 (2016)
Oxidative Medicine and Cellular Longevity
DOI: 10.1155/2016/3187560
Popis: Here we have collected evidence suggesting that chronic changes in the NO homeostasis and the rise of reactive oxygen species bioavailability can contribute to cell dysfunction in Leber’s hereditary optic neuropathy (LHON) patients. We report that peripheral blood mononuclear cells (PBMCs), derived from a female LHON patient with bilateral reduced vision and carrying the pathogenic mutation 11778/ND4, display increased levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS), as revealed by flow cytometry, fluorometric measurements of nitrite/nitrate, and 3-nitrotyrosine immunodetection. Moreover, viability assays with the tetrazolium dye MTT showed that lymphoblasts from the same patient are more sensitive to prolonged NO exposure, leading to cell death. Taken together these findings suggest that oxidative and nitrosative stress cooperatively play an important role in driving LHON pathology when excess NO remains available over time in the cell environment.
Databáze: OpenAIRE
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