Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs
| Autor: | Mikhail Matrosovich, Gaelle Kuntz-Simon, Marek J. Slomka, Chiara Chiapponi, Willie Loeffen, Peter M. H. Heegaard, Michel Bublot, Rebecca A. Gardner, Stephen Essen, Sharon M. Brookes, Alejandro Núñez, Ian H. Brown, Amanda Hanna, Richard M. Irvine, Jaime Maldonado Garcia, Lars Erik Larsen, Bethany J. Nash, Emanuela Foni, Bhudipa Choudhury, Fanny Garcon, Derek Clifford, Jill Banks, Kristien Van Reeth, Stéphane Quéguiner |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Pathology/Histopathology
Swine Viral pathogenesis viruses Respiratory System Public Health and Epidemiology/Infectious Diseases lcsh:Medicine Chick Embryo Virus Replication medicine.disease_cause Disease Outbreaks Influenza A Virus H1N1 Subtype acute-phase protein c-reactive protein Pandemic Influenza A virus receptor-binding properties lcsh:Science Antigens Viral Swine Diseases Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction avian influenza-viruses experimental-infection Immunohistochemistry Virology/Virus Evolution and Symbiosis Virology & Molecular Biology Virology/Viral Replication and Gene Regulation Virology/Animal Models of Infection Research Article Infectious Diseases/Epidemiology and Control of Infectious Diseases Hemagglutinins Viral Molecular Biology/Molecular Evolution interspecies transmission Viral quasispecies Biology Virology/Emerging Viral Diseases Virus Viral Matrix Proteins strains Orthomyxoviridae Infections SDG 3 - Good Health and Well-being Immunology/Immunity to Infections Influenza Human Infectious Diseases/Viral Infections medicine Animals Humans Veterinary Sciences hemagglutinin Base Sequence lcsh:R Sequence Analysis DNA Virology Virologie & Moleculaire Biologie mdck cells Viral replication Viral Receptor Mutation Immunology/Immune Response Tissue tropism lcsh:Q ferrets |
| Zdroj: | PLoS ONE 5 (2010) 2 PLoS ONE, Vol 5, Iss 2, p e9068 (2010) Brookes, S M, Nunez, A, Choudhury, B, Matrosovich, M, Essen, S C, Clifford, D, Slomka, M J, Kuntz-Simon, G, Garcon, F, Nash, B, Hanna, A, Heegaard, P M H, Queguiner, S, Bublot, M, Garcia, J M, Gardner, R, Foni, E, Loeffen, W, Larsen, L E, Reeth, K V, Banks, J, Irvine, R M & Brown, I H 2010, ' Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs ', P L o S One, vol. 5, no. 2, pp. 1-9 . https://doi.org/10.1371/journal.pone.0009068 PLoS ONE, 5(2) PLoS ONE PLOS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0009068 |
| Popis: | The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1], [2]. Two of the three pandemics in the last century resulted in the spread of virus to pigs (H1N1, 1918; H3N2, 1968) with subsequent independent establishment and evolution within swine worldwide [3]. A key public and veterinary health consideration in the context of the evolving pandemic is whether the H1N1/09 virus could become established in pig populations [4]. We performed an infection and transmission study in pigs with A/California/07/09. In combination, clinical, pathological, modified influenza A matrix gene real time RT-PCR and viral genomic analyses have shown that infection results in the induction of clinical signs, viral pathogenesis restricted to the respiratory tract, infection dynamics consistent with endemic strains of influenza A in pigs, virus transmissibility between pigs and virus-host adaptation events. Our results demonstrate that extant H1N1/09 is fully capable of becoming established in global pig populations. We also show the roles of viral receptor specificity in both transmission and tissue tropism. Remarkably, following direct inoculation of pigs with virus quasispecies differing by amino acid substitutions in the haemagglutinin receptor-binding site, only virus with aspartic acid at position 225 (225D) was detected in nasal secretions of contact infected pigs. In contrast, in lower respiratory tract samples from directly inoculated pigs, with clearly demonstrable pulmonary pathology, there was apparent selection of a virus variant with glycine (225G). These findings provide potential clues to the existence and biological significance of viral receptor-binding variants with 225D and 225G during the 1918 pandemic [5]. |
| Databáze: | OpenAIRE |
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