Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs

Autor: Mikhail Matrosovich, Gaelle Kuntz-Simon, Marek J. Slomka, Chiara Chiapponi, Willie Loeffen, Peter M. H. Heegaard, Michel Bublot, Rebecca A. Gardner, Stephen Essen, Sharon M. Brookes, Alejandro Núñez, Ian H. Brown, Amanda Hanna, Richard M. Irvine, Jaime Maldonado Garcia, Lars Erik Larsen, Bethany J. Nash, Emanuela Foni, Bhudipa Choudhury, Fanny Garcon, Derek Clifford, Jill Banks, Kristien Van Reeth, Stéphane Quéguiner
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Pathology/Histopathology
Swine
Viral pathogenesis
viruses
Respiratory System
Public Health and Epidemiology/Infectious Diseases
lcsh:Medicine
Chick Embryo
Virus Replication
medicine.disease_cause
Disease Outbreaks
Influenza A Virus
H1N1 Subtype
acute-phase protein
c-reactive protein
Pandemic
Influenza A virus
receptor-binding properties
lcsh:Science
Antigens
Viral
Swine Diseases
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
avian influenza-viruses
experimental-infection
Immunohistochemistry
Virology/Virus Evolution and Symbiosis
Virology & Molecular Biology
Virology/Viral Replication and Gene Regulation
Virology/Animal Models of Infection
Research Article
Infectious Diseases/Epidemiology and Control of Infectious Diseases
Hemagglutinins
Viral
Molecular Biology/Molecular Evolution
interspecies transmission
Viral quasispecies
Biology
Virology/Emerging Viral Diseases
Virus
Viral Matrix Proteins
strains
Orthomyxoviridae Infections
SDG 3 - Good Health and Well-being
Immunology/Immunity to Infections
Influenza
Human
Infectious Diseases/Viral Infections
medicine
Animals
Humans
Veterinary Sciences
hemagglutinin
Base Sequence
lcsh:R
Sequence Analysis
DNA
Virology
Virologie & Moleculaire Biologie
mdck cells
Viral replication
Viral Receptor
Mutation
Immunology/Immune Response
Tissue tropism
lcsh:Q
ferrets
Zdroj: PLoS ONE 5 (2010) 2
PLoS ONE, Vol 5, Iss 2, p e9068 (2010)
Brookes, S M, Nunez, A, Choudhury, B, Matrosovich, M, Essen, S C, Clifford, D, Slomka, M J, Kuntz-Simon, G, Garcon, F, Nash, B, Hanna, A, Heegaard, P M H, Queguiner, S, Bublot, M, Garcia, J M, Gardner, R, Foni, E, Loeffen, W, Larsen, L E, Reeth, K V, Banks, J, Irvine, R M & Brown, I H 2010, ' Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs ', P L o S One, vol. 5, no. 2, pp. 1-9 . https://doi.org/10.1371/journal.pone.0009068
PLoS ONE, 5(2)
PLoS ONE
PLOS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0009068
Popis: The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1], [2]. Two of the three pandemics in the last century resulted in the spread of virus to pigs (H1N1, 1918; H3N2, 1968) with subsequent independent establishment and evolution within swine worldwide [3]. A key public and veterinary health consideration in the context of the evolving pandemic is whether the H1N1/09 virus could become established in pig populations [4]. We performed an infection and transmission study in pigs with A/California/07/09. In combination, clinical, pathological, modified influenza A matrix gene real time RT-PCR and viral genomic analyses have shown that infection results in the induction of clinical signs, viral pathogenesis restricted to the respiratory tract, infection dynamics consistent with endemic strains of influenza A in pigs, virus transmissibility between pigs and virus-host adaptation events. Our results demonstrate that extant H1N1/09 is fully capable of becoming established in global pig populations. We also show the roles of viral receptor specificity in both transmission and tissue tropism. Remarkably, following direct inoculation of pigs with virus quasispecies differing by amino acid substitutions in the haemagglutinin receptor-binding site, only virus with aspartic acid at position 225 (225D) was detected in nasal secretions of contact infected pigs. In contrast, in lower respiratory tract samples from directly inoculated pigs, with clearly demonstrable pulmonary pathology, there was apparent selection of a virus variant with glycine (225G). These findings provide potential clues to the existence and biological significance of viral receptor-binding variants with 225D and 225G during the 1918 pandemic [5].
Databáze: OpenAIRE
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