Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia
Autor: | Tanja Lunić, Biljana Dj. Božić Nedeljković, Aneta Sabovljevic, Uroš Gašić, Sonja Duletić-Laušević, Marko Sabovljevic, Bojan D.-J. Božić, Mariana Oalđe, Marija R. Mandić |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Antioxidant
Tyrosinase medicine.medical_treatment Flavonoid Ethyl acetate Apoptosis Biochemistry Antioxidants Terpene chemistry.chemical_compound 0302 clinical medicine Coumarins Breast Tumors Medicine and Health Sciences Hydroxybenzoates Nonvascular Plants chemistry.chemical_classification 0303 health sciences Multidisciplinary biology Traditional medicine Cell Death Organic Compounds Eukaryota Biological activity Proteases Plants Enzymes Chemistry Oncology Cell Processes 030220 oncology & carcinogenesis Physical Sciences Medicine Serbia Research Article Science Bryophyta Nitric Oxide Cell Line 03 medical and health sciences Phenols Breast Cancer medicine Mosses Humans 030304 developmental biology Flavonoids Ethanol Ciliata Terpenes Plant Extracts Soxhlet extractor Organic Chemistry Chemical Compounds Organisms Biology and Life Sciences Proteins Cancers and Neoplasms Cell Biology Fibroblasts biology.organism_classification Triterpenes chemistry Alcohols Enzymology Serine Proteases |
Zdroj: | PLoS ONE, Vol 16, Iss 2, p e0246810 (2021) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H. ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss extracts in vitro, which allows the design of new experiments aimed at detecting and characterizing bioactive compounds of the extracts and additionally elucidate detailed mechanisms of their effects. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |