Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia

Autor: Tanja Lunić, Biljana Dj. Božić Nedeljković, Aneta Sabovljevic, Uroš Gašić, Sonja Duletić-Laušević, Marko Sabovljevic, Bojan D.-J. Božić, Mariana Oalđe, Marija R. Mandić
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Antioxidant
Tyrosinase
medicine.medical_treatment
Flavonoid
Ethyl acetate
Apoptosis
Biochemistry
Antioxidants
Terpene
chemistry.chemical_compound
0302 clinical medicine
Coumarins
Breast Tumors
Medicine and Health Sciences
Hydroxybenzoates
Nonvascular Plants
chemistry.chemical_classification
0303 health sciences
Multidisciplinary
biology
Traditional medicine
Cell Death
Organic Compounds
Eukaryota
Biological activity
Proteases
Plants
Enzymes
Chemistry
Oncology
Cell Processes
030220 oncology & carcinogenesis
Physical Sciences
Medicine
Serbia
Research Article
Science
Bryophyta
Nitric Oxide
Cell Line
03 medical and health sciences
Phenols
Breast Cancer
medicine
Mosses
Humans
030304 developmental biology
Flavonoids
Ethanol
Ciliata
Terpenes
Plant Extracts
Soxhlet extractor
Organic Chemistry
Chemical Compounds
Organisms
Biology and Life Sciences
Proteins
Cancers and Neoplasms
Cell Biology
Fibroblasts
biology.organism_classification
Triterpenes
chemistry
Alcohols
Enzymology
Serine Proteases
Zdroj: PLoS ONE, Vol 16, Iss 2, p e0246810 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H. ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss extracts in vitro, which allows the design of new experiments aimed at detecting and characterizing bioactive compounds of the extracts and additionally elucidate detailed mechanisms of their effects.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje