Effects of boron deficiency in cell suspension cultures of Populus alba L
| Autor: | Tadashi Ishii, Koichi Kakegawa, Toshiro Matsunaga |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
inorganic chemicals
Arabinose Dimer Carbohydrates chemistry.chemical_element Plant Science Biology Polysaccharide Cell wall chemistry.chemical_compound Cell Wall Polysaccharides Sugar Boron Cells Cultured chemistry.chemical_classification General Medicine Kinetics Populus Uronic Acids chemistry Biochemistry Cell culture Composition (visual arts) Agronomy and Crop Science Cell Division |
| Zdroj: | Plant Cell Reports. 23:573-578 |
| ISSN: | 1432-203X 0721-7714 |
| Popis: | Cell suspension cultures of Populus alba L. (original cells) require at least 10 microM boron for appropriate growth. Using original cells we established a cell line, T-5B, which can grow in a medium containing low levels of boron (5 microM). The level of boron localized in the cell walls of T-5B cells was one-half that found in the cell walls of original cells maintained in medium containing 100 microM boron, and the level of the rhamnogalacturonan II dimer, cross-linked by a borate ester, also decreased in the former. The sugar composition of whole cell walls of the T-5B cell line was similar that of the original cells, however pectic polysaccharides composed of arabinose or galacturonic acid were easily extracted from T-5B cell walls with 50 mM trans-1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid. Our results suggest that boron deficiency causes a weakening of the interaction among pectic polysaccharides due to a decrease in boron-rhamnogalacturonanII cross-linkage. |
| Databáze: | OpenAIRE |
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