Effects of boron deficiency in cell suspension cultures of Populus alba L

Autor: Tadashi Ishii, Koichi Kakegawa, Toshiro Matsunaga
Rok vydání: 2004
Předmět:
Zdroj: Plant Cell Reports. 23:573-578
ISSN: 1432-203X
0721-7714
Popis: Cell suspension cultures of Populus alba L. (original cells) require at least 10 microM boron for appropriate growth. Using original cells we established a cell line, T-5B, which can grow in a medium containing low levels of boron (5 microM). The level of boron localized in the cell walls of T-5B cells was one-half that found in the cell walls of original cells maintained in medium containing 100 microM boron, and the level of the rhamnogalacturonan II dimer, cross-linked by a borate ester, also decreased in the former. The sugar composition of whole cell walls of the T-5B cell line was similar that of the original cells, however pectic polysaccharides composed of arabinose or galacturonic acid were easily extracted from T-5B cell walls with 50 mM trans-1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid. Our results suggest that boron deficiency causes a weakening of the interaction among pectic polysaccharides due to a decrease in boron-rhamnogalacturonanII cross-linkage.
Databáze: OpenAIRE