Biosensor zebrafish provide new insights into potential health effects of environmental estrogens
| Autor: | Tetsuhiro Kudoh, Okhyun Lee, Aya Takesono, Masazumi Tada, Charles R. Tyler |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Health Toxicology and Mutagenesis Transgene Green Fluorescent Proteins Estrogen receptor EDCs Biosensing Techniques 010501 environmental sciences Endocrine Disruptors biosensor 01 natural sciences Green fluorescent protein Animals Genetically Modified 03 medical and health sciences Internal medicine medicine Animals 14. Life underwater endocrine-disrupting chemicals Zebrafish News | Science Selections 030304 developmental biology 0105 earth and related environmental sciences Regulation of gene expression 0303 health sciences biology Research Systems Biology Public Health Environmental and Occupational Health Estrogens Animal Models biology.organism_classification upstream activation sequence Environmental Estrogen Cell biology Endocrinology ER Gene Expression Regulation Estrogen Gal4 UAS Otic vesicle hormones hormone substitutes and hormone antagonists estrogen receptor Transgenics |
| Zdroj: | Environmental Health Perspectives |
| ISSN: | 1552-9924 |
| Popis: | Background: Environmental estrogens alter hormone signaling in the body that can induce reproductive abnormalities in both humans and wildlife. Available testing systems for estrogens are focused on specific systems such as reproduction. Crucially, however, the potential for significant health impacts of environmental estrogen exposures on a variety of body systems may have been overlooked. Objective: Our aim was to develop and apply a sensitive transgenic zebrafish model to assess real-time effects of environmental estrogens on signaling mechanisms in a whole body system for use in integrated health assessments. Methods: We created a novel transgenic biosensor zebrafish containing an estrogen-inducible promoter derived with multiple tandem estrogen responsive elements (EREs) and a Gal4ff-UAS system for enhanced response sensitivity. Results: Using our novel estrogen-responsive transgenic (TG) zebrafish, we identified target tissues for environmental estrogens; these tissues have very high sensitivity even at environmentally relevant concentrations. Exposure of the TG fish to estrogenic endocrine-disrupting chemicals (EDCs) induced specific expression of green fluorescent protein (GFP) in a wide variety of tissues including the liver, heart, skeletal muscle, otic vesicle, forebrain, lateral line, and ganglions, most of which have not been established previously as targets for estrogens in fish. Furthermore, we found that different EDCs induced GFP expression with different tissue response patterns and time trajectories, suggesting different potential health effects. Conclusion: We have developed a powerful new model for understanding toxicological effects, mechanisms, and health impacts of environmental estrogens in vertebrates. |
| Databáze: | OpenAIRE |
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