Biosensor zebrafish provide new insights into potential health effects of environmental estrogens

Autor: Tetsuhiro Kudoh, Okhyun Lee, Aya Takesono, Masazumi Tada, Charles R. Tyler
Rok vydání: 2011
Předmět:
medicine.medical_specialty
medicine.drug_class
Health
Toxicology and Mutagenesis
Transgene
Green Fluorescent Proteins
Estrogen receptor
EDCs
Biosensing Techniques
010501 environmental sciences
Endocrine Disruptors
biosensor
01 natural sciences
Green fluorescent protein
Animals
Genetically Modified
03 medical and health sciences
Internal medicine
medicine
Animals
14. Life underwater
endocrine-disrupting chemicals
Zebrafish
News | Science Selections
030304 developmental biology
0105 earth and related environmental sciences
Regulation of gene expression
0303 health sciences
biology
Research
Systems Biology
Public Health
Environmental and Occupational Health
Estrogens
Animal Models
biology.organism_classification
upstream activation sequence
Environmental Estrogen
Cell biology
Endocrinology
ER
Gene Expression Regulation
Estrogen
Gal4
UAS
Otic vesicle
hormones
hormone substitutes
and hormone antagonists
estrogen receptor
Transgenics
Zdroj: Environmental Health Perspectives
ISSN: 1552-9924
Popis: Background: Environmental estrogens alter hormone signaling in the body that can induce reproductive abnormalities in both humans and wildlife. Available testing systems for estrogens are focused on specific systems such as reproduction. Crucially, however, the potential for significant health impacts of environmental estrogen exposures on a variety of body systems may have been overlooked. Objective: Our aim was to develop and apply a sensitive transgenic zebrafish model to assess real-time effects of environmental estrogens on signaling mechanisms in a whole body system for use in integrated health assessments. Methods: We created a novel transgenic biosensor zebrafish containing an estrogen-inducible promoter derived with multiple tandem estrogen responsive elements (EREs) and a Gal4ff-UAS system for enhanced response sensitivity. Results: Using our novel estrogen-responsive transgenic (TG) zebrafish, we identified target tissues for environmental estrogens; these tissues have very high sensitivity even at environmentally relevant concentrations. Exposure of the TG fish to estrogenic endocrine-disrupting chemicals (EDCs) induced specific expression of green fluorescent protein (GFP) in a wide variety of tissues including the liver, heart, skeletal muscle, otic vesicle, forebrain, lateral line, and ganglions, most of which have not been established previously as targets for estrogens in fish. Furthermore, we found that different EDCs induced GFP expression with different tissue response patterns and time trajectories, suggesting different potential health effects. Conclusion: We have developed a powerful new model for understanding toxicological effects, mechanisms, and health impacts of environmental estrogens in vertebrates.
Databáze: OpenAIRE
Externí odkaz: