NCBP3 positively impacts mRNA biogenesis

Autor: Yuhui Dou, Hua Jiang, Kelly R. Molloy, Wiebke Manuela Schulze, Hervé Le Hir, Torben Heick Jensen, Manfred Schmid, Claudia Iasillo, Isabelle Barbosa, Stephen Cusack, John LaCava
Přispěvatelé: Damage and Repair in Cancer Development and Cancer Treatment (DARE), Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aarhus University [Aarhus], Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS Paris, European Molecular Biology Laboratory [Grenoble] (EMBL), Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, Rockefeller University [New York]
Rok vydání: 2020
Předmět:
CAP-BINDING COMPLEX
Polyadenylation
Transcription
Genetic

AcademicSubjects/SCI00010
[SDV]Life Sciences [q-bio]
RNA Stability
Active Transport
Cell Nucleus

PROTEIN
SPLICEOSOME
RNA polymerase II
Biology
NUCLEAR EXPORT
RNA Transport
03 medical and health sciences
Exon
0302 clinical medicine
Transcription (biology)
BAC TRANSGENEOMICS
Genetics
Humans
RNA
Messenger

Nuclear export signal
Molecular Biology
Nuclear Cap-Binding Protein Complex
030304 developmental biology
GENE-EXPRESSION
JUNCTION CORE COMPLEX
Cell Nucleus
0303 health sciences
Messenger RNA
RNA
RNA-Binding Proteins
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology

ASSOCIATION
Exons
Cell biology
Gene Expression Regulation
RNA Cap-Binding Proteins
HUMAN TREX COMPLEX
biology.protein
Exon junction complex
RNA Polymerase II
DECAY
030217 neurology & neurosurgery
Transcription Factors
Zdroj: Nucleic Acids Research
Dou, Y, Barbosa, I, Jiang, H, Iasillo, C, Molloy, K R, Schulze, W M, Cusack, S, Schmid, M, Le Hir, H, LaCava, J & Jensen, T H 2020, ' NCBP3 positively impacts mRNA biogenesis ', Nucleic Acids Research, vol. 48, no. 18, pp. 10413-10427 . https://doi.org/10.1093/nar/gkaa744
Nucleic Acids Research, 48(18), 10413-10427. Oxford University Press
Nucleic Acids Research, Oxford University Press, 2020, 48 (18), pp.10413-10427. ⟨10.1093/nar/gkaa744⟩
ISSN: 1362-4962
0305-1048
DOI: 10.1093/nar/gkaa744
Popis: The nuclear Cap-Binding Complex (CBC), consisting of Nuclear Cap-Binding Protein 1 (NCBP1) and 2 (NCBP2), associates with the nascent 5′cap of RNA polymerase II transcripts and impacts RNA fate decisions. Recently, the C17orf85 protein, also called NCBP3, was suggested to form an alternative CBC by replacing NCBP2. However, applying protein–protein interaction screening of NCBP1, 2 and 3, we find that the interaction profile of NCBP3 is distinct. Whereas NCBP1 and 2 identify known CBC interactors, NCBP3 primarily interacts with components of the Exon Junction Complex (EJC) and the TRanscription and EXport (TREX) complex. NCBP3-EJC association in vitro and in vivo requires EJC core integrity and the in vivo RNA binding profiles of EJC and NCBP3 overlap. We further show that NCBP3 competes with the RNA degradation factor ZC3H18 for binding CBC-bound transcripts, and that NCBP3 positively impacts the nuclear export of polyadenylated RNAs and the expression of large multi-exonic transcripts. Collectively, our results place NCBP3 with the EJC and TREX complexes in supporting mRNA expression.
Databáze: OpenAIRE
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