NCBP3 positively impacts mRNA biogenesis
| Autor: | Yuhui Dou, Hua Jiang, Kelly R. Molloy, Wiebke Manuela Schulze, Hervé Le Hir, Torben Heick Jensen, Manfred Schmid, Claudia Iasillo, Isabelle Barbosa, Stephen Cusack, John LaCava |
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| Přispěvatelé: | Damage and Repair in Cancer Development and Cancer Treatment (DARE), Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aarhus University [Aarhus], Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS Paris, European Molecular Biology Laboratory [Grenoble] (EMBL), Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, Rockefeller University [New York] |
| Rok vydání: | 2020 |
| Předmět: |
CAP-BINDING COMPLEX
Polyadenylation Transcription Genetic AcademicSubjects/SCI00010 [SDV]Life Sciences [q-bio] RNA Stability Active Transport Cell Nucleus PROTEIN SPLICEOSOME RNA polymerase II Biology NUCLEAR EXPORT RNA Transport 03 medical and health sciences Exon 0302 clinical medicine Transcription (biology) BAC TRANSGENEOMICS Genetics Humans RNA Messenger Nuclear export signal Molecular Biology Nuclear Cap-Binding Protein Complex 030304 developmental biology GENE-EXPRESSION JUNCTION CORE COMPLEX Cell Nucleus 0303 health sciences Messenger RNA RNA RNA-Binding Proteins [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology ASSOCIATION Exons Cell biology Gene Expression Regulation RNA Cap-Binding Proteins HUMAN TREX COMPLEX biology.protein Exon junction complex RNA Polymerase II DECAY 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Nucleic Acids Research Dou, Y, Barbosa, I, Jiang, H, Iasillo, C, Molloy, K R, Schulze, W M, Cusack, S, Schmid, M, Le Hir, H, LaCava, J & Jensen, T H 2020, ' NCBP3 positively impacts mRNA biogenesis ', Nucleic Acids Research, vol. 48, no. 18, pp. 10413-10427 . https://doi.org/10.1093/nar/gkaa744 Nucleic Acids Research, 48(18), 10413-10427. Oxford University Press Nucleic Acids Research, Oxford University Press, 2020, 48 (18), pp.10413-10427. ⟨10.1093/nar/gkaa744⟩ |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkaa744 |
| Popis: | The nuclear Cap-Binding Complex (CBC), consisting of Nuclear Cap-Binding Protein 1 (NCBP1) and 2 (NCBP2), associates with the nascent 5′cap of RNA polymerase II transcripts and impacts RNA fate decisions. Recently, the C17orf85 protein, also called NCBP3, was suggested to form an alternative CBC by replacing NCBP2. However, applying protein–protein interaction screening of NCBP1, 2 and 3, we find that the interaction profile of NCBP3 is distinct. Whereas NCBP1 and 2 identify known CBC interactors, NCBP3 primarily interacts with components of the Exon Junction Complex (EJC) and the TRanscription and EXport (TREX) complex. NCBP3-EJC association in vitro and in vivo requires EJC core integrity and the in vivo RNA binding profiles of EJC and NCBP3 overlap. We further show that NCBP3 competes with the RNA degradation factor ZC3H18 for binding CBC-bound transcripts, and that NCBP3 positively impacts the nuclear export of polyadenylated RNAs and the expression of large multi-exonic transcripts. Collectively, our results place NCBP3 with the EJC and TREX complexes in supporting mRNA expression. |
| Databáze: | OpenAIRE |
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