Glutamatergic cells in the periaqueductal gray matter mediate sensory inputs after bladder stimulation in freely moving rats

Autor: Gommert van Koeveringe, Harry W.M. Steinbusch, Celine Meriaux, Aryo Zare, Ali Jahanshahi
Přispěvatelé: Neurochirurgie, RS: MHeNs - R3 - Neuroscience, Urologie, Promovendi MHN, Faculteit FHML Centraal, MUMC+: MA Urologie (3)
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
BARRINGTONS NUCLEUS
Urology
Glutamate decarboxylase
Urinary Bladder
Glutamic Acid
Urination
Stimulation
Periaqueductal gray
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Glutamatergic
0302 clinical medicine
RAPHE MAGNUS
Internal medicine
MICTURITION REFLEX
Medicine
PROJECTION NEURONS
Animals
Periaqueductal Gray
Neurons
Afferent
NITRIC-OXIDE SYNTHASE
Neurotransmitter
Nucleus raphe magnus
Tyrosine hydroxylase
business.industry
CAT
Glutamic acid
FOS EXPRESSION
Electric Stimulation
Electrodes
Implanted
Rats
030104 developmental biology
Endocrinology
chemistry
Models
Animal
ANESTHETIZED RATS
business
URINARY-BLADDER
Proto-Oncogene Proteins c-fos
030217 neurology & neurosurgery
neurotransmitter
DEEP BRAIN-STIMULATION
Zdroj: International Journal of Urology, 25(6), 621-626. Wiley-Blackwell
ISSN: 1442-2042
0919-8172
Popis: Objectives To determine the phenotype of the ventrolateral part of the periaqueductal gray matter neurons after bladder stimulation. Methods In the experimental group, electrical stimulation of the bladder was carried out under freely moving condition by a bipolar stimulation electrode implanted in the bladder wall. Thereafter, the brain sections were processed for immunohistochemical analysis using antibodies against c-Fos (neuronal activation marker) together with one of the following: tyrosine hydroxylase (dopaminergic cell marker), vesicular glutamate transporter (glutamatergic cell marker), serotonin, glutamate decarboxylase (glutamate decarboxylase 67, gamma-aminobutyric acid cell marker) and neuronal nitric oxide synthase. We used design-based confocal stereological analysis to quantify the immunohistochemically stained sections. Results A significant increase in the number of c-Fos-positive cells in the ventrolateral part of the periaqueductal gray matter after stimulation was found. Furthermore, the ratio of c-Fos cells double labeled with vesicular glutamate transporter was significantly higher in the ventrolateral part of the periaqueductal gray matter region in the stimulated compared with the sham group. Quantitative analysis of the other four cell types did not show any significant difference. Conclusion These findings suggest that glutamatergic neurotransmission in the ventrolateral part of the periaqueductal gray matter is seemingly the main pathway to be activated after receiving sensory signals from the bladder.
Databáze: OpenAIRE
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