Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina

Autor: Alex Gobbi, Mónica Oyuela Aguilar, Lars Hestbjerg Hansen, Liliana Semorile, Patrick Denis Browne, Mariano Pistorio, Lea Ellegaard-Jensen
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Fungal Structure
Climate
Biología
Biodiversity
Wine
Plant Science
Cultivar and soil characteristics
Biochemistry
ARBUSCULAR MYCORRHIZAL FUNGI
Soil
Agricultural Soil Science
Medicine and Health Sciences
Vitis
Flowering Plants
2. Zero hunger
0303 health sciences
Rhizosphere
Multidisciplinary
Geography
Ecology
biology
Microbiota
Alcoholic Beverages
Influence of vintage
VITIS-VINIFERA L
RNA GENES DIFFERENTIATION
Eukaryota
Agriculture
Genomics
Plants
Bioquímica y Biología Molecular
Nucleic acids
Ribosomal RNA
Medical Microbiology
SOLUBILIZATION
Medicine
Grapevine
Proteobacteria
Research Article
Cell biology
Cellular structures and organelles
Firmicutes
Science
Argentina
Soil Science
Microbial communities
Microbial Genomics
Mycology
Microbiology
Vineyard
Beverages
03 medical and health sciences
Plant-Environment Interactions
Botany
Genetics
Vineyard location
Non-coding RNA
Nutrition
030304 developmental biology
Terroir
Bacteria
030306 microbiology
Plant Ecology
Ecology and Environmental Sciences
Fungi
Organisms
Biology and Life Sciences
Bacteroidetes
15. Life on land
biology.organism_classification
Diet
Microbial population biology
Earth Sciences
SOIL BACTERIAL DIVERSITY
RNA
Microbiome
TERROIR
Ribosomes
Zdroj: CIC Digital (CICBA)
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
Aguilar, M O, Gobbi, A, Browne, P D, Ellegaard-Jensen, L, Hansen, L H, Semorile, L & Pistorio, M 2020, ' Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina ', PLOS ONE, vol. 15, no. 12, e0243848 . https://doi.org/10.1371/journal.pone.0243848
PLoS ONE
SEDICI (UNLP)
Universidad Nacional de La Plata
instacron:UNLP
PLoS ONE, Vol 15, Iss 12, p e0243848 (2020)
Aguilar, M O, Gobbi, A, Browne, P D, Ellegaard-Jensen, L, Hansen, L H, Semorile, L & Pistorio, M 2020, ' Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina ', PLoS ONE, vol. 15, no. 12, 0243848 . https://doi.org/10.1371/journal.pone.0243848
DOI: 10.1371/journal.pone.0243848
Popis: Soil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities have increased dramatically in recent years, establishing links between wine specificity and, environmental and viticultural factors, which are framed into the elusive terroir concept. Given the diverse and complex role these factors play on microbial soil structuring of agricultural crops, the main aim of this study is to evaluate how external factors, such as vintage, vineyard location, cultivar and soil characteristics, may affect the diversity of the microbial communities present. Additionally, we aim to compare the influence these factors have on the structuring of bacterial and fungal populations associated with Malbec grapevine rhizosphere with that of the more widespread Cabernet Sauvignon grapevine cultivar. Samples were taken from Malbec and Cabernet Sauvignon cultivars from two different vineyards in the San Juan Province of Argentina. Total DNA extracts from the rhizosphere soil samples were sequenced using Illumina’s Miseq technology, targeting the V3-V4 hypervariable 16S rRNA region in prokaryotes and the ITS1 region in yeasts. The major bacterial taxa identified were Proteobacteria, Bacteroidetes and Firmicutes, while the major fungal taxa were Ascomycetes, Basidiomycetes, Mortierellomycetes and a low percentage of Glomeromycetes. Significant differences in microbial community composition were found between vintages and vineyard locations, whose soils showed variances in pH, organic matter, and content of carbon, nitrogen, and absorbable phosphorus.
Instituto de Biotecnología y Biología Molecular
Databáze: OpenAIRE
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