Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
| Autor: | Alex Gobbi, Mónica Oyuela Aguilar, Lars Hestbjerg Hansen, Liliana Semorile, Patrick Denis Browne, Mariano Pistorio, Lea Ellegaard-Jensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Fungal Structure
Climate Biología Biodiversity Wine Plant Science Cultivar and soil characteristics Biochemistry ARBUSCULAR MYCORRHIZAL FUNGI Soil Agricultural Soil Science Medicine and Health Sciences Vitis Flowering Plants 2. Zero hunger 0303 health sciences Rhizosphere Multidisciplinary Geography Ecology biology Microbiota Alcoholic Beverages Influence of vintage VITIS-VINIFERA L RNA GENES DIFFERENTIATION Eukaryota Agriculture Genomics Plants Bioquímica y Biología Molecular Nucleic acids Ribosomal RNA Medical Microbiology SOLUBILIZATION Medicine Grapevine Proteobacteria Research Article Cell biology Cellular structures and organelles Firmicutes Science Argentina Soil Science Microbial communities Microbial Genomics Mycology Microbiology Vineyard Beverages 03 medical and health sciences Plant-Environment Interactions Botany Genetics Vineyard location Non-coding RNA Nutrition 030304 developmental biology Terroir Bacteria 030306 microbiology Plant Ecology Ecology and Environmental Sciences Fungi Organisms Biology and Life Sciences Bacteroidetes 15. Life on land biology.organism_classification Diet Microbial population biology Earth Sciences SOIL BACTERIAL DIVERSITY RNA Microbiome TERROIR Ribosomes |
| Zdroj: | CIC Digital (CICBA) Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA Aguilar, M O, Gobbi, A, Browne, P D, Ellegaard-Jensen, L, Hansen, L H, Semorile, L & Pistorio, M 2020, ' Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina ', PLOS ONE, vol. 15, no. 12, e0243848 . https://doi.org/10.1371/journal.pone.0243848 PLoS ONE SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP PLoS ONE, Vol 15, Iss 12, p e0243848 (2020) Aguilar, M O, Gobbi, A, Browne, P D, Ellegaard-Jensen, L, Hansen, L H, Semorile, L & Pistorio, M 2020, ' Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina ', PLoS ONE, vol. 15, no. 12, 0243848 . https://doi.org/10.1371/journal.pone.0243848 |
| DOI: | 10.1371/journal.pone.0243848 |
| Popis: | Soil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities have increased dramatically in recent years, establishing links between wine specificity and, environmental and viticultural factors, which are framed into the elusive terroir concept. Given the diverse and complex role these factors play on microbial soil structuring of agricultural crops, the main aim of this study is to evaluate how external factors, such as vintage, vineyard location, cultivar and soil characteristics, may affect the diversity of the microbial communities present. Additionally, we aim to compare the influence these factors have on the structuring of bacterial and fungal populations associated with Malbec grapevine rhizosphere with that of the more widespread Cabernet Sauvignon grapevine cultivar. Samples were taken from Malbec and Cabernet Sauvignon cultivars from two different vineyards in the San Juan Province of Argentina. Total DNA extracts from the rhizosphere soil samples were sequenced using Illumina’s Miseq technology, targeting the V3-V4 hypervariable 16S rRNA region in prokaryotes and the ITS1 region in yeasts. The major bacterial taxa identified were Proteobacteria, Bacteroidetes and Firmicutes, while the major fungal taxa were Ascomycetes, Basidiomycetes, Mortierellomycetes and a low percentage of Glomeromycetes. Significant differences in microbial community composition were found between vintages and vineyard locations, whose soils showed variances in pH, organic matter, and content of carbon, nitrogen, and absorbable phosphorus. Instituto de Biotecnología y Biología Molecular |
| Databáze: | OpenAIRE |
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