High rates of apoptosis visualized in the symbiont-bearing gills of deep-sea Bathymodiolus mussels

Autor: Ann C. Andersen, François H. Lallier, Sébastien Duperron, Bérénice Piquet, Bruce Shillito
Přispěvatelé: Adaptation et Biologie des Invertébrés en Conditions Extrêmes (ABICE), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Adaptation et diversité en milieu marin (ADMM), Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
0106 biological sciences
Gill
Gills
Hemocytes
Respiratory System
Bathymodiolus
Marine and Aquatic Sciences
Apoptosis
01 natural sciences
White Blood Cells
Cell Signaling
Animal Cells
Medicine and Health Sciences
Mussels
Animal Anatomy
0303 health sciences
Multidisciplinary
Cell Death
biology
Eukaryota
Signaling Cascades
Mytilus
Cold seeps
Hydrothermal vents
Cell Processes
Medicine
Anatomy
Cellular Types
Marine Geology
Research Article
Signal Transduction
Hydrothermal vent
Bivalves
animal structures
Science
Immune Cells
Immunology
Zoology
010603 evolutionary biology
03 medical and health sciences
Apoptotic signaling cascade
Symbiosis
Species Specificity
Gammaproteobacteria
Animals
14. Life underwater
030304 developmental biology
Blood Cells
010604 marine biology & hydrobiology
Bacteriocyte
fungi
Organisms
Biology and Life Sciences
Cell Biology
Molluscs
Mussel
biology.organism_classification
Invertebrates
Cold seep
Bivalvia
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
Species Interactions
030104 developmental biology
Aquatic Respiratory Anatomy
Earth Sciences
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology
environment/Symbiosis
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2019, 14 (2), pp.e0211499. ⟨10.1371/journal.pone.0211499⟩
PLoS ONE, 2019, 14 (2), pp.e0211499. ⟨10.1371/journal.pone.0211499⟩
Plos One (1932-6203) (Public Library Science), 2019-02, Vol. 14, N. 2, P. e0211499 (21p.)
PLoS ONE, Vol 14, Iss 2, p e0211499 (2019)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0211499⟩
Popis: Symbiosis between Bathymodiolus and Gammaproteobacteria enables these deep-sea mussels to live in toxic environments like hydrothermal vents and cold seeps. The quantity of endosymbionts within the gill-bacteriocytes appears to vary according to the hosts environment. We investigated the hypothesis of a control of the endosymbionts density by apoptosis, a programmed cell death. We used fluorometric TUNEL-method and active Caspase-3-targeting antibodies to visualize and quantify apoptotic cells in mussel gills. To avoid artefacts due to depressurization upon specimen recovery from the deep-sea, we compared the apoptotic rates between mussels recovered unpressurised, versus mussels recovered in a pressure-maintaining device, in two species from hydrothermal vents on the Mid-Atlantic Ridge: Bathymodiolus azoricus and B. puteoserpentis. Our results show that pressurized recovery had no significant effect on the apoptotic rate in the gill filaments. Apoptotic levels were highest in the ciliated zone and in the circulating hemocytes, compared to the bacteriocyte zone. Apoptotic gill-cells in B. aff. boomerang from the pockmarks off the Gulf of Guinea, show similar distribution patterns. Deep-sea symbiotic mussels have much higher rates of apoptosis in their gills than the coastal mussel Mytilus edulis without chemolithoautotrophic symbionts. We discuss how apoptosis might be one of the mechanisms that contribute to the adaptation of deep-sea mussels to toxic environments and/or to symbiosis.
Databáze: OpenAIRE
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