High rates of apoptosis visualized in the symbiont-bearing gills of deep-sea Bathymodiolus mussels
| Autor: | Ann C. Andersen, François H. Lallier, Sébastien Duperron, Bérénice Piquet, Bruce Shillito |
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| Přispěvatelé: | Adaptation et Biologie des Invertébrés en Conditions Extrêmes (ABICE), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Adaptation et diversité en milieu marin (ADMM), Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
0106 biological sciences Gill Gills Hemocytes Respiratory System Bathymodiolus Marine and Aquatic Sciences Apoptosis 01 natural sciences White Blood Cells Cell Signaling Animal Cells Medicine and Health Sciences Mussels Animal Anatomy 0303 health sciences Multidisciplinary Cell Death biology Eukaryota Signaling Cascades Mytilus Cold seeps Hydrothermal vents Cell Processes Medicine Anatomy Cellular Types Marine Geology Research Article Signal Transduction Hydrothermal vent Bivalves animal structures Science Immune Cells Immunology Zoology 010603 evolutionary biology 03 medical and health sciences Apoptotic signaling cascade Symbiosis Species Specificity Gammaproteobacteria Animals 14. Life underwater 030304 developmental biology Blood Cells 010604 marine biology & hydrobiology Bacteriocyte fungi Organisms Biology and Life Sciences Cell Biology Molluscs Mussel biology.organism_classification Invertebrates Cold seep Bivalvia [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology Species Interactions 030104 developmental biology Aquatic Respiratory Anatomy Earth Sciences [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2019, 14 (2), pp.e0211499. ⟨10.1371/journal.pone.0211499⟩ PLoS ONE, 2019, 14 (2), pp.e0211499. ⟨10.1371/journal.pone.0211499⟩ Plos One (1932-6203) (Public Library Science), 2019-02, Vol. 14, N. 2, P. e0211499 (21p.) PLoS ONE, Vol 14, Iss 2, p e0211499 (2019) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0211499⟩ |
| Popis: | Symbiosis between Bathymodiolus and Gammaproteobacteria enables these deep-sea mussels to live in toxic environments like hydrothermal vents and cold seeps. The quantity of endosymbionts within the gill-bacteriocytes appears to vary according to the hosts environment. We investigated the hypothesis of a control of the endosymbionts density by apoptosis, a programmed cell death. We used fluorometric TUNEL-method and active Caspase-3-targeting antibodies to visualize and quantify apoptotic cells in mussel gills. To avoid artefacts due to depressurization upon specimen recovery from the deep-sea, we compared the apoptotic rates between mussels recovered unpressurised, versus mussels recovered in a pressure-maintaining device, in two species from hydrothermal vents on the Mid-Atlantic Ridge: Bathymodiolus azoricus and B. puteoserpentis. Our results show that pressurized recovery had no significant effect on the apoptotic rate in the gill filaments. Apoptotic levels were highest in the ciliated zone and in the circulating hemocytes, compared to the bacteriocyte zone. Apoptotic gill-cells in B. aff. boomerang from the pockmarks off the Gulf of Guinea, show similar distribution patterns. Deep-sea symbiotic mussels have much higher rates of apoptosis in their gills than the coastal mussel Mytilus edulis without chemolithoautotrophic symbionts. We discuss how apoptosis might be one of the mechanisms that contribute to the adaptation of deep-sea mussels to toxic environments and/or to symbiosis. |
| Databáze: | OpenAIRE |
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