Dietary curcumin supplementation does not alter peripheral blood mononuclear cell responses to exertional heat stress
| Autor: | Trevor Gillum, Zach J Schall, Peter A Falgiano, Matthew R. Kuennen, Harrison R. Strag |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Hyperthermia medicine.medical_specialty Curcumin Adolescent Physiology medicine.medical_treatment Physical Exertion HSP72 Heat-Shock Proteins Inflammation Heat Stress Disorders Peripheral blood mononuclear cell Monocytes Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) Internal medicine medicine Humans Orthopedics and Sports Medicine Heat shock business.industry Anti-Inflammatory Agents Non-Steroidal NF-kappa B Public Health Environmental and Occupational Health 030229 sport sciences General Medicine medicine.disease Hsp70 Toll-Like Receptor 4 030104 developmental biology Endocrinology Cytokine chemistry Dietary Supplements Myeloid Differentiation Factor 88 TLR4 Female medicine.symptom business Biomarkers Heat-Shock Response |
| Zdroj: | European Journal of Applied Physiology. 118:2707-2717 |
| ISSN: | 1439-6327 1439-6319 |
| DOI: | 10.1007/s00421-018-3998-5 |
| Popis: | Curcumin reduces gut barrier damage and plasma cytokine responses to exertional heat stress. However, the role of peripheral blood mononuclear cell (PBMC) in this response remains unclear. This work investigated the effect of 3 days of 500 mg/day dietary curcumin supplementation on PBMC responses to exertional heat stress in non-heat acclimated humans. Eight participants ran (65% VO2max) for 60 min in an environmental chamber (37 °C/25% RH) two times (curcumin/placebo). Blood samples were collected pre, post, 1 h post, and 4 h post-exercise. PBMC were isolated from blood samples and the protein content of markers along the TLR4 signaling pathway (TLR4, MyD88, pNF-κB, NF-κB), indicators of cellular energy status (SIRT1 and p-AMPK), and mediators of cellular heat shock response (pHSF-1 and HSP70) were examined with Western blot. Data were analyzed with two-way (condition × time) RM-ANOVAs with Newman–Keuls post hocs. As compared to placebo, curcumin did not alter protein expression in PBMC (p > 0.05). However, in both study conditions at 1 h post-reductions were noted in TLR 4 (− 21.5%; p = 0.03), HSP70 (− 11.0%; p = 0.04), pAMPK (− 48.5%; p |
| Databáze: | OpenAIRE |
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