Dietary curcumin supplementation does not alter peripheral blood mononuclear cell responses to exertional heat stress

Autor: Trevor Gillum, Zach J Schall, Peter A Falgiano, Matthew R. Kuennen, Harrison R. Strag
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Hyperthermia
medicine.medical_specialty
Curcumin
Adolescent
Physiology
medicine.medical_treatment
Physical Exertion
HSP72 Heat-Shock Proteins
Inflammation
Heat Stress Disorders
Peripheral blood mononuclear cell
Monocytes
Young Adult
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Physiology (medical)
Internal medicine
medicine
Humans
Orthopedics and Sports Medicine
Heat shock
business.industry
Anti-Inflammatory Agents
Non-Steroidal
NF-kappa B
Public Health
Environmental and Occupational Health
030229 sport sciences
General Medicine
medicine.disease
Hsp70
Toll-Like Receptor 4
030104 developmental biology
Endocrinology
Cytokine
chemistry
Dietary Supplements
Myeloid Differentiation Factor 88
TLR4
Female
medicine.symptom
business
Biomarkers
Heat-Shock Response
Zdroj: European Journal of Applied Physiology. 118:2707-2717
ISSN: 1439-6327
1439-6319
DOI: 10.1007/s00421-018-3998-5
Popis: Curcumin reduces gut barrier damage and plasma cytokine responses to exertional heat stress. However, the role of peripheral blood mononuclear cell (PBMC) in this response remains unclear. This work investigated the effect of 3 days of 500 mg/day dietary curcumin supplementation on PBMC responses to exertional heat stress in non-heat acclimated humans. Eight participants ran (65% VO2max) for 60 min in an environmental chamber (37 °C/25% RH) two times (curcumin/placebo). Blood samples were collected pre, post, 1 h post, and 4 h post-exercise. PBMC were isolated from blood samples and the protein content of markers along the TLR4 signaling pathway (TLR4, MyD88, pNF-κB, NF-κB), indicators of cellular energy status (SIRT1 and p-AMPK), and mediators of cellular heat shock response (pHSF-1 and HSP70) were examined with Western blot. Data were analyzed with two-way (condition × time) RM-ANOVAs with Newman–Keuls post hocs. As compared to placebo, curcumin did not alter protein expression in PBMC (p > 0.05). However, in both study conditions at 1 h post-reductions were noted in TLR 4 (− 21.5%; p = 0.03), HSP70 (− 11.0%; p = 0.04), pAMPK (− 48.5%; p
Databáze: OpenAIRE
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