ESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra da Estrela, Portugal

Autor: Cátia Oliveira, Rúben Fernandes, Cristina Prudêncio, Paulo Tavares-Ratado, Cândida T. Tomaz, Paula Amador
Přispěvatelé: uBibliorum, Repositório Científico do Instituto Politécnico do Porto, Instituto de Investigação e Inovação em Saúde
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Medicine (General)
Drug Resistance
Microbial / genetics
beta-Lactamases / genetics
Cephalosporin
Antibiotics
β-lactamases
resistance mechanisms
Resistance mechanisms
medicine.disease_cause
Germ-Line Mutation / genetics
Genotype
Portugal / epidemiology
AmpC
Escherichia coli Infections
ß-lactamases
0303 health sciences
β lactamases
Drug Resistance
Microbial
General Medicine
Phenotype
3. Good health
Escherichia coli Infections / epidemiology
medicine.drug
Bacterial Proteins / genetics
medicine.drug_class
Biology
Escherichia coli Infections / drug therapy
Article
beta-Lactamases
Microbiology
03 medical and health sciences
R5-920
Cephalosporins / pharmacology
Bacterial Proteins
Clavulanic acid
medicine
Escherichia coli
Humans
Gene
Germ-Line Mutation
Serra da Estrela
030304 developmental biology
Portugal
ESBL
030306 microbiology
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Escherichia coli / genetics
beta-Lactamases / analysis
Cephalosporins
Cephalosporins / therapeutic use
Bacterial Proteins / analysis
Zdroj: Medicina
Volume 55
Issue 6
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Medicina; Volume 55; Issue 6; Pages: 272
Medicina, Vol 55, Iss 6, p 272 (2019)
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação
ISSN: 1010-660X
DOI: 10.3390/medicina55060272
Popis: Background and Objectives: Given the considerable spatial, temporal, and ecological factors, heterogeneity, which affects emergency response, persistence, and dissemination of genetic determinants that confer microorganisms their resistance to antibiotics, several authors claim that antibiotics&rsquo
resistance must be perceived as an ecological problem. The aim of this study was to determine the prevalence of broad-spectrum bla genes, not only Extended-spectrum &beta
lactamases (ESBL) but also AmpC-types, in clinical strains of Escherichia coli isolated from Portugal (in the highest region of the country, Serra da Estrela) to disclose susceptibility profiles among different genotypes, and to compare the distribution of bla genes expressing broad-spectrum enzymes. Materials and Methods: Clinical strains of Escherichia coli presenting resistance to third generation (3G) cephalosporins and susceptibility to inhibition by clavulanic acid were studied by means of phenotypic and molecular profiling techniques for encoding &beta
lactamases genes. Results: Strains were mainly isolated from hospital populations (97%). Molecular analysis enabled the detection of 49 bla genes, in which 55% (27/49) were identified as blaOXA-1-like, 33% (16/49) as blaCTX-M-group-1, 10% (5/49) as blaTEM, and 2% (1/49) were identified as genes blaCIT (AmpC). Among all blaOXA-1-like detected, about 59% of strains expressed at least another bla gene. Co-production of &beta
lactamases was observed in 40% of strains, with the co-production of CTX-M group 1 and OXA-1-like occurring as the most frequent. Conclusions: This is the first study using microorganisms isolated from native people from the highest Portuguese mountain regions, showing an unprecedent high prevalence of genes blaOXA-1-like in this country.
Databáze: OpenAIRE
Externí odkaz: