ESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra da Estrela, Portugal
| Autor: | Cátia Oliveira, Rúben Fernandes, Cristina Prudêncio, Paulo Tavares-Ratado, Cândida T. Tomaz, Paula Amador |
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| Přispěvatelé: | uBibliorum, Repositório Científico do Instituto Politécnico do Porto, Instituto de Investigação e Inovação em Saúde |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Medicine (General)
Drug Resistance Microbial / genetics beta-Lactamases / genetics Cephalosporin Antibiotics β-lactamases resistance mechanisms Resistance mechanisms medicine.disease_cause Germ-Line Mutation / genetics Genotype Portugal / epidemiology AmpC Escherichia coli Infections ß-lactamases 0303 health sciences β lactamases Drug Resistance Microbial General Medicine Phenotype 3. Good health Escherichia coli Infections / epidemiology medicine.drug Bacterial Proteins / genetics medicine.drug_class Biology Escherichia coli Infections / drug therapy Article beta-Lactamases Microbiology 03 medical and health sciences R5-920 Cephalosporins / pharmacology Bacterial Proteins Clavulanic acid medicine Escherichia coli Humans Gene Germ-Line Mutation Serra da Estrela 030304 developmental biology Portugal ESBL 030306 microbiology biochemical phenomena metabolism and nutrition bacterial infections and mycoses Escherichia coli / genetics beta-Lactamases / analysis Cephalosporins Cephalosporins / therapeutic use Bacterial Proteins / analysis |
| Zdroj: | Medicina Volume 55 Issue 6 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Medicina; Volume 55; Issue 6; Pages: 272 Medicina, Vol 55, Iss 6, p 272 (2019) Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação |
| ISSN: | 1010-660X |
| DOI: | 10.3390/medicina55060272 |
| Popis: | Background and Objectives: Given the considerable spatial, temporal, and ecological factors, heterogeneity, which affects emergency response, persistence, and dissemination of genetic determinants that confer microorganisms their resistance to antibiotics, several authors claim that antibiotics&rsquo resistance must be perceived as an ecological problem. The aim of this study was to determine the prevalence of broad-spectrum bla genes, not only Extended-spectrum &beta lactamases (ESBL) but also AmpC-types, in clinical strains of Escherichia coli isolated from Portugal (in the highest region of the country, Serra da Estrela) to disclose susceptibility profiles among different genotypes, and to compare the distribution of bla genes expressing broad-spectrum enzymes. Materials and Methods: Clinical strains of Escherichia coli presenting resistance to third generation (3G) cephalosporins and susceptibility to inhibition by clavulanic acid were studied by means of phenotypic and molecular profiling techniques for encoding &beta lactamases genes. Results: Strains were mainly isolated from hospital populations (97%). Molecular analysis enabled the detection of 49 bla genes, in which 55% (27/49) were identified as blaOXA-1-like, 33% (16/49) as blaCTX-M-group-1, 10% (5/49) as blaTEM, and 2% (1/49) were identified as genes blaCIT (AmpC). Among all blaOXA-1-like detected, about 59% of strains expressed at least another bla gene. Co-production of &beta lactamases was observed in 40% of strains, with the co-production of CTX-M group 1 and OXA-1-like occurring as the most frequent. Conclusions: This is the first study using microorganisms isolated from native people from the highest Portuguese mountain regions, showing an unprecedent high prevalence of genes blaOXA-1-like in this country. |
| Databáze: | OpenAIRE |
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