Ectopic B7-H4-Ig expression attenuates concanavalin A-induced hepatic injury
| Autor: | Huan Xiao, Cong-Yi Wang, Shu-Hua Zheng, Fang Zheng, Jing Lü, Guo-Yan Hu, Wei Liu, Heng Yang, Chun-lei Yuan, Feili Gong, Jun-Fa Xu |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Recombinant Fusion Proteins Immunology Gene Expression Biology Gene delivery Interferon-gamma Mice Necrosis Internal medicine medicine Concanavalin A Immunology and Allergy Animals Humans Interferon gamma Aspartate Aminotransferases Hepatitis Liver injury Mice Inbred BALB C Genetic transfer Gene Transfer Techniques Alanine Transaminase Genetic Therapy V-Set Domain-Containing T-Cell Activation Inhibitor 1 medicine.disease Survival Analysis Immunoglobulin Fc Fragments Interleukin-10 Endocrinology Cytokine Liver Immunoglobulin G biology.protein B7-1 Antigen Leukocytes Mononuclear Interleukin-2 Ectopic expression Interleukin-4 Chemical and Drug Induced Liver Injury medicine.drug |
| Zdroj: | Clinical immunology (Orlando, Fla.). 136(1) |
| ISSN: | 1521-7035 |
| Popis: | Previous studies demonstrate that both membrane B7-H4 and B7-H4-Ig fusion protein could inhibit T-cell responses. In the present study, we explored the potential effect of B7-H4-Ig on liver injury in a hepatitis mouse model induced by concanavalin A (ConA). A B7-H4-Ig construct was introduced into animals by the hydrodynamic gene delivery approach. It was found that ectopic expression of B7-H4-Ig could inhibit ConA-induced elevation of serum levels of ALT and AST, suppress liver necrosis and even mortality of mice. Furthermore, we observed that pretreatment of B7-H4-Ig dramatically decreased serum levels and the expression of mRNA for IL-2, IFN-gamma and IL-4, but increased IL-10 in ConA-treated mice. Our results suggest that B7-H4-Ig may protect animals from liver injury induced by ConA, which could be associated with reduced serum levels for IL-2, IFN-gamma and IL-4 as well as enhanced IL-10 production. |
| Databáze: | OpenAIRE |
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