Effect of nutrition and Enteromyxum leei infection on gilthead sea bream Sparus aurata intestinal carbohydrate distribution
Autor: | Itziar Estensoro, María J. Redondo, Beatriz Salesa, Savasidam Kaushik, Ariadna Sitjà-Bobadilla, Jaume Pérez-Sánchez |
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Přispěvatelé: | Institute of Aquaculture Torre la Sal (IATS), Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA) |
Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
myxozoa
[SDV]Life Sciences [q-bio] Fish Diseases chemistry.chemical_compound Agglutinin Intestinal Mucosa myxosporea ComputingMilieux_MISCELLANEOUS 2. Zero hunger 0303 health sciences biology 04 agricultural and veterinary sciences Anatomy Fish oil 3. Good health Intestines medicine.anatomical_structure Plant protein histochemistry parasite Carbohydrate Metabolism Animal Nutritional Physiological Phenomena replacement diet Parasitic Diseases Animal gobblet cell Nutritional Status Periodic acid–Schiff stain Aquatic Science 03 medical and health sciences Fish Oils Dietary Fats Unsaturated mucin medicine Animals Plant Oils [INFO]Computer Science [cs] Ecology Evolution Behavior and Systematics 030304 developmental biology Goblet cell Mucin Mucins Lectin Animal Feed Molecular biology Sea Bream Diet Sialic acid chemistry 040102 fisheries biology.protein 0401 agriculture forestry and fisheries lectin |
Zdroj: | Diseases of Aquatic Organisms Diseases of Aquatic Organisms, Inter Research, 2012, 100 (1), pp.29-42. ⟨10.3354/dao02486⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
ISSN: | 0177-5103 |
DOI: | 10.3354/dao02486⟩ |
Popis: | The effect of a practical plant protein-based diet containing vegetable oils (VO) as the major lipid source on the mucosal carbohydrate pattern of the intestine was studied in gilthead sea bream Sparus aurata challenged with the myxosporean parasite Enteromyxum leei. Fish fed for 9 mo either a fish oil (FO) diet or a blend of VO at 66% of replacement (66VO diet) were exposed to parasite-contaminated water effluent. Samples of the anterior, middle and posterior intestine (AI, MI and PI, respectively) were obtained for parasite diagnosis and histochemistry. Fish were categorised as control (C, not exposed), early (E) or late (L) infected. Mucin and lectin histochemistry was applied to detect the different types of mucins and sialic acid in goblet cells (GC), the brush border and enterocytes. The number of GC stained with periodic acid Schiff (PAS), alcian blue (AB), aldehyde fuchsin-alcian blue (AF-AB), for the detection of neutral, acidic, sulphated and carboxylic mucins, and with the lectin Sambucus nigra agglutinin (SNA), were counted in digital images. The 66VO diet produced a significant decrease of GC with neutral and acidic mucins in the AI and MI, and also of those with carboxylic mucins and sialic acid in the MI. Sulphated mucins and sialic acid were less abundant in the AI than in the MI and PI in the C- 66VO treatment. E. leei infection had a strong effect on the number of GC, as E and L infected fish had a significant decrease of GC positive for all the stains versus C fish in PI. Time and diet effects were also observed, since the lowest values were mostly registered in E-66VO fish in PI. In conclusion, though GC depletion was mainly induced by enteromyxosis, an effect of the diet was also observed. Thus, the diet can be a predisposing factor that worsens the disease course. © Inter-Research 2012. EU through project AQUAMAX FOOD-CT-2006-16249, EU through project ARRAINA KBBE-2011-288925, Spanish Ministry of Science and Innovation (MICINN) AGL2009-13282-C02-01, Generalitat Valenciana PROMETEO 2010/006, MICINN, CSIC. |
Databáze: | OpenAIRE |
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