Effect of nutrition and Enteromyxum leei infection on gilthead sea bream Sparus aurata intestinal carbohydrate distribution

Autor: Itziar Estensoro, María J. Redondo, Beatriz Salesa, Savasidam Kaushik, Ariadna Sitjà-Bobadilla, Jaume Pérez-Sánchez
Přispěvatelé: Institute of Aquaculture Torre la Sal (IATS), Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
myxozoa
[SDV]Life Sciences [q-bio]
Fish Diseases
chemistry.chemical_compound
Agglutinin
Intestinal Mucosa
myxosporea
ComputingMilieux_MISCELLANEOUS
2. Zero hunger
0303 health sciences
biology
04 agricultural and veterinary sciences
Anatomy
Fish oil
3. Good health
Intestines
medicine.anatomical_structure
Plant protein
histochemistry
parasite
Carbohydrate Metabolism
Animal Nutritional Physiological Phenomena
replacement diet
Parasitic Diseases
Animal
gobblet cell
Nutritional Status
Periodic acid–Schiff stain
Aquatic Science
03 medical and health sciences
Fish Oils
Dietary Fats
Unsaturated
mucin
medicine
Animals
Plant Oils
[INFO]Computer Science [cs]
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Goblet cell
Mucin
Mucins
Lectin
Animal Feed
Molecular biology
Sea Bream
Diet
Sialic acid
chemistry
040102 fisheries
biology.protein
0401 agriculture
forestry
and fisheries
lectin
Zdroj: Diseases of Aquatic Organisms
Diseases of Aquatic Organisms, Inter Research, 2012, 100 (1), pp.29-42. ⟨10.3354/dao02486⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 0177-5103
DOI: 10.3354/dao02486⟩
Popis: The effect of a practical plant protein-based diet containing vegetable oils (VO) as the major lipid source on the mucosal carbohydrate pattern of the intestine was studied in gilthead sea bream Sparus aurata challenged with the myxosporean parasite Enteromyxum leei. Fish fed for 9 mo either a fish oil (FO) diet or a blend of VO at 66% of replacement (66VO diet) were exposed to parasite-contaminated water effluent. Samples of the anterior, middle and posterior intestine (AI, MI and PI, respectively) were obtained for parasite diagnosis and histochemistry. Fish were categorised as control (C, not exposed), early (E) or late (L) infected. Mucin and lectin histochemistry was applied to detect the different types of mucins and sialic acid in goblet cells (GC), the brush border and enterocytes. The number of GC stained with periodic acid Schiff (PAS), alcian blue (AB), aldehyde fuchsin-alcian blue (AF-AB), for the detection of neutral, acidic, sulphated and carboxylic mucins, and with the lectin Sambucus nigra agglutinin (SNA), were counted in digital images. The 66VO diet produced a significant decrease of GC with neutral and acidic mucins in the AI and MI, and also of those with carboxylic mucins and sialic acid in the MI. Sulphated mucins and sialic acid were less abundant in the AI than in the MI and PI in the C- 66VO treatment. E. leei infection had a strong effect on the number of GC, as E and L infected fish had a significant decrease of GC positive for all the stains versus C fish in PI. Time and diet effects were also observed, since the lowest values were mostly registered in E-66VO fish in PI. In conclusion, though GC depletion was mainly induced by enteromyxosis, an effect of the diet was also observed. Thus, the diet can be a predisposing factor that worsens the disease course. © Inter-Research 2012.
EU through project AQUAMAX FOOD-CT-2006-16249, EU through project ARRAINA KBBE-2011-288925, Spanish Ministry of Science and Innovation (MICINN) AGL2009-13282-C02-01, Generalitat Valenciana PROMETEO 2010/006, MICINN, CSIC.
Databáze: OpenAIRE
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