Mathematical models and fractal analysis for the investigation of the photodynamic inactivation in phytopathogenic microorganisms
| Autor: | Nara C. de Souza, Alessandro S. Kalck, Josmary R. Silva, Maria F. Costa Pedro, Marcos S. Sousa, Kevin F. dos Santos |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antifungal Agents Microorganism 030106 microbiology Microbial Sensitivity Tests Models Biological 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry Humans Photosensitizer Physical and Theoretical Chemistry Photosensitizing Agents Mathematical model Chemistry Acridine orange Fungi Surfaces and Interfaces General Medicine Contamination Fractal analysis Acridine Orange Fractals 030104 developmental biology Photochemotherapy Sunlight Molecular oxygen Biological system Function (biology) Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 171:285-290 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2018.07.019 |
| Popis: | The increasing and indiscriminate use of pesticides may lead to the intoxication and contamination of the environment and foods. In addition, pesticides can cause fungal resistance promoting the selection of resistant phytopathogenic fungi. This is a problem in the agricultural and human health areas, which leads to a need for developing new methodologies to address this problem. Photodynamic inactivation is a promising strategy involving the association of a photosensitizer (PS), light, and molecular oxygen to inhibit the growth of microorganisms. In this work, the PS acridine orange (AO) was deposited using the spray layer-by-layer technique. The effectiveness of the method was evaluated by the analysis of the growth evolution of the colonies as a function of the amount of PS layers applied in field in the presence of sunlight. Image processing and analysis of the fractal theory were used to evaluate the growth of the colonies. The results revealed that AO is a candidate PS for use in field. It was possible to observe the reduction of the growth dynamics of the colonies with the increase of the number of PS layers. The parameters related to the fractality of the system were described by mathematical models of the fractal growth of interfaces. The knowledge of these parameters can help to identify new strategies for the control of phytopathogenic microorganisms that directly affect agricultural production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect