Avian osteopetrosis virus induces proliferation of cultured bone cells

Autor:	Emmett V. Schmidt, Ralph E. Smith
Rok vydání:	1981
Předmět:	Periosteum Avian Leukosis Virus Cell Chick Embryo Biology Alkaline Phosphatase Isozyme Virology Molecular biology Virus Clone Cells Isoenzymes Limb bud medicine.anatomical_structure Bone cell medicine Animals Alkaline phosphatase Proteoglycans Specific activity Collagen Cell Division Cells Cultured
Zdroj:	Virology. 111:275-282
ISSN:	0042-6822
DOI:	10.1016/0042-6822(81)90672-3
Popis:	Bone cells isolated from the periosteum of 8-day embryonic chick limb buds were infected with an avian leukosis virus (MAV-2(O)). Monolayers of cultured periosteal cells contained bone-specific alkaline phosphatase. After infection with MAV-2(O), the specific activity of this isoenzyme was increased on a per cell basis. MAV-2(O)-infected bone cells were then cloned in methylcellulose culture. These clones grew at a higher rate than did clones of uninfected cells. The limb bud cell assay for MAV-2(O) described in this report is the first developed for measuring the proliferative effects of an avian leukosis virus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60fde5999d389d7177146a9bb1577f97 https://doi.org/10.1016/0042-6822(81)90672-3 Zobrazit plný text záznamu