Obsah

Avian osteopetrosis virus induces proliferation of cultured bone cells

Autor: Emmett V. Schmidt, Ralph E. Smith
Rok vydání: 1981
Předmět:
Zdroj: Virology. 111:275-282
ISSN: 0042-6822
DOI: 10.1016/0042-6822(81)90672-3
Popis: Bone cells isolated from the periosteum of 8-day embryonic chick limb buds were infected with an avian leukosis virus (MAV-2(O)). Monolayers of cultured periosteal cells contained bone-specific alkaline phosphatase. After infection with MAV-2(O), the specific activity of this isoenzyme was increased on a per cell basis. MAV-2(O)-infected bone cells were then cloned in methylcellulose culture. These clones grew at a higher rate than did clones of uninfected cells. The limb bud cell assay for MAV-2(O) described in this report is the first developed for measuring the proliferative effects of an avian leukosis virus.
Databáze: OpenAIRE
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