Avian osteopetrosis virus induces proliferation of cultured bone cells
Autor: | Emmett V. Schmidt, Ralph E. Smith |
---|---|
Rok vydání: | 1981 |
Předmět: | |
Zdroj: | Virology. 111:275-282 |
ISSN: | 0042-6822 |
DOI: | 10.1016/0042-6822(81)90672-3 |
Popis: | Bone cells isolated from the periosteum of 8-day embryonic chick limb buds were infected with an avian leukosis virus (MAV-2(O)). Monolayers of cultured periosteal cells contained bone-specific alkaline phosphatase. After infection with MAV-2(O), the specific activity of this isoenzyme was increased on a per cell basis. MAV-2(O)-infected bone cells were then cloned in methylcellulose culture. These clones grew at a higher rate than did clones of uninfected cells. The limb bud cell assay for MAV-2(O) described in this report is the first developed for measuring the proliferative effects of an avian leukosis virus. |
Databáze: | OpenAIRE |
Externí odkaz: |
Pro tento záznam nejsou dostupné žádné jednotky.