Tissue dependent variations of DNA methylation and endoreduplication levels during tomato fruit development and ripening
Autor: | Stéphane Maury, Giovanni Bernacchia, L. Stammitti-Bert, A. How Kit, Philippe Gallusci, Emeline Teyssier, Dominique Rolin |
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Přispěvatelé: | Station de physiologie végétale, Institut National de la Recherche Agronomique (INRA), Università degli Studi di Ferrara (UniFE), Université d'Orléans (UO) |
Rok vydání: | 2008 |
Předmět: |
0106 biological sciences
Methyltransferase ADN Flowers Plant Science Biology Genes Plant CYTOSINE METHYLEE 01 natural sciences TISSUS LOCULAIRE ENDOREDUPLICATION DNA methyltranferase · Fruit pericarp · Locular tissue · Methylcytosine [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics Cytosine 03 medical and health sciences GELEE Solanum lycopersicum Gene Expression Regulation Plant Gene Duplication Genetics BIOLOGIE DU DEVELOPPEMENT Endoreduplication DNA (Cytosine-5-)-Methyltransferases Epigenetics Repetitive Sequences Nucleic Acid 030304 developmental biology BIOLOGIE VEGETALE 0303 health sciences Ploidies food and beverages Ripening Methylation DNA Methylation Molecular biology genomic DNA PERICARPE Organ Specificity Fruit DNA methylation METHYLTRANSFERASE Ploidy 010606 plant biology & botany |
Zdroj: | Planta Planta, Springer Verlag, 2008, 228 (3), pp.391-399. ⟨10.1007/s00425-008-0743-z⟩ |
ISSN: | 1432-2048 0032-0935 |
DOI: | 10.1007/s00425-008-0743-z |
Popis: | International audience; Tomato fruit cells are characterized by a strong increase in nuclear ploidy during fruit development. Average ploidy levels increased to similar levels (above 50C) in two distinct fruit tissues, pericarp and locular tissue. However, ploidy profiles differed significantly between these two tissues suggesting a tissue-specific control of endoreduplication in tomato fruit. To determine possible relationships between endoreduplication and epigenetic mechanisms, the methylation status of genomic DNA from pericarp and locular tissue of tomato fruit was analysed. Pericarp genomic DNA was characterized by an increase of CG and/or CNG methylation at the 5S and 18S rDNA loci and at gyspsy-like retrotransposon sequences during fruit growth. A sharp decrease of the global DNA methylation level together with a reduction of methylation at the rDNA loci was also observed in pericarp during fruit ripening. Inversely, no major variation of DNA methylation either global or locus-specific, was observed in locular tissue. Thus, tissue-specific variations of DNA methylation are unlikely to be triggered by the induction of endoreduplication in fruit tissues, but may reflect tissue-specific ploidy profiles. Expression analysis of eight putative tomato DNA methyltransferases encoding genes showed that one chromomethylase (CMT) and two rearranged methyltransferases (DRMs) are preferentially expressed in the pericarp during fruit growth and could be involved in the locus-specific increase of methylation observed at this developmental phase in the pericarp. |
Databáze: | OpenAIRE |
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