Tissue dependent variations of DNA methylation and endoreduplication levels during tomato fruit development and ripening

Autor: Stéphane Maury, Giovanni Bernacchia, L. Stammitti-Bert, A. How Kit, Philippe Gallusci, Emeline Teyssier, Dominique Rolin
Přispěvatelé: Station de physiologie végétale, Institut National de la Recherche Agronomique (INRA), Università degli Studi di Ferrara (UniFE), Université d'Orléans (UO)
Rok vydání: 2008
Předmět:
0106 biological sciences
Methyltransferase
ADN
Flowers
Plant Science
Biology
Genes
Plant
CYTOSINE METHYLEE
01 natural sciences
TISSUS LOCULAIRE
ENDOREDUPLICATION
DNA methyltranferase · Fruit pericarp · Locular tissue · Methylcytosine
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
Cytosine
03 medical and health sciences
GELEE
Solanum lycopersicum
Gene Expression Regulation
Plant
Gene Duplication
Genetics
BIOLOGIE DU DEVELOPPEMENT
Endoreduplication
DNA (Cytosine-5-)-Methyltransferases
Epigenetics
Repetitive Sequences
Nucleic Acid
030304 developmental biology
BIOLOGIE VEGETALE
0303 health sciences
Ploidies
food and beverages
Ripening
Methylation
DNA Methylation
Molecular biology
genomic DNA
PERICARPE
Organ Specificity
Fruit
DNA methylation
METHYLTRANSFERASE
Ploidy
010606 plant biology & botany
Zdroj: Planta
Planta, Springer Verlag, 2008, 228 (3), pp.391-399. ⟨10.1007/s00425-008-0743-z⟩
ISSN: 1432-2048
0032-0935
DOI: 10.1007/s00425-008-0743-z
Popis: International audience; Tomato fruit cells are characterized by a strong increase in nuclear ploidy during fruit development. Average ploidy levels increased to similar levels (above 50C) in two distinct fruit tissues, pericarp and locular tissue. However, ploidy profiles differed significantly between these two tissues suggesting a tissue-specific control of endoreduplication in tomato fruit. To determine possible relationships between endoreduplication and epigenetic mechanisms, the methylation status of genomic DNA from pericarp and locular tissue of tomato fruit was analysed. Pericarp genomic DNA was characterized by an increase of CG and/or CNG methylation at the 5S and 18S rDNA loci and at gyspsy-like retrotransposon sequences during fruit growth. A sharp decrease of the global DNA methylation level together with a reduction of methylation at the rDNA loci was also observed in pericarp during fruit ripening. Inversely, no major variation of DNA methylation either global or locus-specific, was observed in locular tissue. Thus, tissue-specific variations of DNA methylation are unlikely to be triggered by the induction of endoreduplication in fruit tissues, but may reflect tissue-specific ploidy profiles. Expression analysis of eight putative tomato DNA methyltransferases encoding genes showed that one chromomethylase (CMT) and two rearranged methyltransferases (DRMs) are preferentially expressed in the pericarp during fruit growth and could be involved in the locus-specific increase of methylation observed at this developmental phase in the pericarp.
Databáze: OpenAIRE
Externí odkaz: