In vivo systemic chlorogenic acid therapy under diabetic conditions: Wound healing effects and cytotoxicity/genotoxicity profile

Autor: Nihal Y. Gul, Mine Sibel Gurun, Zulfiye Gul, Ayse Topal, Ozge Turacozen, Sevda Inan, Betul Cam Etoz, Nilufer Cinkilic, Sedef Ziyanok, Sibel Tas, Musa Ozgur Ozyigit, Deniz Bagdas
Přispěvatelé: Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi., Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı., Uludağ Üniversitesi/Fen ve Edebiyat Fakültesi/Biyoloji Bölümü., Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı., Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı., Bağdaş, Deniz, Etöz, Betül Cam, Gül, Zülfiye, Ziyanok, Sedef, İnan, Sevda, Turaçözen, Özge, Gül, Nihal, Topal, Ayşe B., Çinkılıç, Nilüfer, Taş, Sibel, Özyiğit, Musa Özgür, Gürün, Mine Sibel, AAR-6478-2021, AAF-9939-2020, AAG-8716-2019, E-3364-2018, ABE-6873-2020, AAH-4272-2021, AAH-2873-2021, AAH-5296-2021
Rok vydání: 2014
Předmět:
Blood Glucose
Male
Antioxidant
Diabetic wound healing
Cytotoxicity
medicine.medical_treatment
Instant coffee
Glucose blood level
Pharmacology
Toxicology
medicine.disease_cause
Biochemistry
Animal tissue
Antioxidants
Rats
Sprague-Dawley
In vivo study
chemistry.chemical_compound
Diabetes mellitus
Nitric-oxide
Malondialdehyde
Pathology
Medicine
Drug safety
Sprague Dawley rat
biology
Experimental diabetes mellitus
Malonaldehyde
General Medicine
Catalase
Glutathione
Hydroxyproline
Rat model
Prooxidant activities
Chlorogenic Acid
Drug mechanism
Histopathology
Food science & technology
Nitric Oxide
Article
Streptozocin
Carlina Oxide
Lonicera
Nitric oxide
Diabetes Mellitus
Experimental
Dna-damage
Superoxide dismutase
Phenethyl ester
Animalia
Animals
Animal model
Animal experiment
Caffeic acid
Wound Healing
Drug effects
Rattus
Animal
business.industry
Superoxide Dismutase
Wound
Nonhuman
Rats
Topical application
Drug efficacy
Oxidative Stress
Glucose
Metabolism
chemistry
biology.protein
Rat
Lipid Peroxidation
Genotoxicity
Wound healing
business
Reactive Oxygen Species
Reactive oxygen metabolite
Controlled study
Oxidative stress
Food Science
DNA Damage
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 81
ISSN: 1873-6351
Popis: Oxidative stress occurs following the impairment of pro-oxidant/antioxidant balance in chronic wounds and leads to harmful delays in healing progress. A fine balance between oxidative stress and endogenous antioxidant defense system may be beneficial for wound healing under redox control. This study tested the hypothesis that oxidative stress in wound area can be controlled with systemic antioxidant therapy and therefore wound healing can be accelerated. We used chlorogenic acid (CGA), a dietary antioxidant, in experimental diabetic wounds that are characterized by delayed healing. Additionally, we aimed to understand possible side effects of CGA on pivotal organs and bone marrow during therapy. Wounds were created on backs of streptozotocin-induced diabetic rats. CGA (50 mg/kg/day) was injected intraperitoneally. Animals were sacrificed on different days. Biochemical and histopathological examinations were performed. Side effects of chronic antioxidant treatment were tested. CGA accelerated wound healing, enhanced hydroxyproline content, decreased malondialdehyde/nitric oxide levels, elevated reduced-glutathione, and did not affect superoxide dismutase/catalase levels in wound bed. While CGA induced side effects such as cyto/genotoxicity, 15 days of treatment attenuated blood glucose levels. CGA decreased lipid peroxidation levels of main organs. This study provides a better understanding for antioxidant intake on diabetic wound repair and possible pro-oxidative effects.
Databáze: OpenAIRE
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